A method for the colorimetric estimation of glycogen with lodine

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Abstract

A rapid, simple, and specific method for the colorimetric estimation of glycogen in concentrations varying from 0.15 to 1 mg/ml with an iodine-iodide reagent in the presence of salts has been studied. It eliminates interference by polysaccharides, which do not develop color in the presence of iodine. This colorimetric procedure can be applied to the estimation of tissue glycogen.

References (17)

  • R. Montgomery

    Arch. Biochem. Biophys

    (1957)
  • C.A. Good et al.

    J. Biol. Chem

    (1933)
  • W.J. Van Wagtendonk et al.

    J. Biol. Chem

    (1946)
  • M. Somogyi
  • S. Hestrin

    J. Biol. Chem

    (1949)
  • M. Swanson

    J. Biol. Chem

    (1948)
  • J.M. Bailey et al.

    J. Biol. Chem

    (1961)
  • M. Dubois et al.

    Anal. Chem

    (1956)
There are more references available in the full text version of this article.

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This investigation was supported in part by a research grant (No. G-3442) from the National Institutes of Health, U. S. Public Health Service, by the Rockefeller Foundation, and by Consejo Nacional de Investigaciones Científicas y Técnicas. The data in this paper are part of a thesis for the degree of Doctor of Chemistry at the Buenos Aires University.

2

Investigator, Instituto Nacional de Microbiología.

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