Elsevier

Analytical Biochemistry

Volume 43, Issue 1, September 1971, Pages 129-133
Analytical Biochemistry

Direct spectrophotometric determination of inorganic sulfide in biological materials and in other complex mixtures

https://doi.org/10.1016/0003-2697(71)90116-3Get rights and content

Abstract

An improved direct spectrophotometric method is described that is suited to determinations of sulfide in enzyme mixtures and in biological materials. The assay is based on the specific incorporation of sulfide into methylene blue in the presence of acidic, N,N-dimethyl-p-phenylenedi-amine and ferric chloride.

The major improvement in the method is achieved by quantitative precipitation of zinc sulfide and removal of the soluble components of the sample. This procedure replaces the cumbersome entrainment of hydrogen sulfide with nitrogen gas prior to addition of the color-forming reagents. This very simple in situ manipulation is efficient in preventing the interfering effects of foreign constituents of the sample medium and contributes to a further increase in the sensitivity of the method.

References (5)

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  • L.M. Siegel

    Anal. Biochem

    (1965)
There are more references available in the full text version of this article.

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