Elsevier

Analytical Biochemistry

Volume 51, Issue 2, February 1973, Pages 429-435
Analytical Biochemistry

Subcellular particles separated through a histochemical reaction

https://doi.org/10.1016/0003-2697(73)90496-XGet rights and content

Abstract

A method is presented for increasing the density of mitochondria in order to separate them more thoroughly from synaptic ending membranes in density-gradient centrifugation. The mixture of particulates is incubated with a histochemical reaction medium for the mitochondrial specific enzyme, succinic dehydrogenase, and then applied to the gradient. The contamination of synaptic ending membranes by mitochondria is greatly reduced thereby, and it is suggested that this sort of procedure may be useful in many cases where particulates are isolated through centrifugation.

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