Preparing nuclei from cells in monolayer cultures suitable for counting and for following synchronized cells through the cell cycle☆
References (8)
- et al.
Exp. Cell Res
(1979) - et al.
J. Natl. Cancer Inst
(1951)
Cited by (121)
Repair pathway for PARP-1 DNA-protein crosslinks
2019, DNA RepairCitation Excerpt :After washing, cells were further incubated as appropriate with MG-132 for a further 23 h. After washing again, cells were incubated until 80% confluence of untreated wells, then nuclei were harvested (triplicate wells for each drug concentration) following cell lysis using hypotonic solution and detergent [50]. Results were expressed as percentage of control cells without any treatment.
XRCC1-mediated repair of strand breaks independent of PNKP binding
2017, DNA RepairCitation Excerpt :After washing as appropriate, growth medium was added. Triplicate wells for each drug concentration were counted by a cell lysis procedure [38,43] when untreated cells were 80% confluent, and results expressed as% control growth. Fold hypersensitivity was determined at IC90 concentrations, the dose required for 90% decrease in cell growth.
Role of the oxidized form of XRCC1 in protection against extreme oxidative stress
2017, Free Radical Biology and MedicineCitation Excerpt :After washing as appropriate, growth medium was added. Triplicate wells for each drug concentration were counted by a cell lysis procedure [30,32] when untreated cells were 80% confluent, and results expressed as % control growth. Fold sensitization was determined at IC90 concentrations, the dose required for 90% decrease in cell growth.
DNA polymerase β-dependent cell survival independent of XRCC1 expression
2015, DNA RepairCitation Excerpt :After washing as needed, growth medium was added and cells further incubated. When untreated cells were 80% confluent, triplicate wells for each drug concentration were counted by a cell lysis procedure as utilized previously [21,22]. Briefly, the cell culture medium was aspirated, and cells washed with 0.9% saline.
- ☆
This investigation was supported by Grant CA-21606, awarded by the National Cancer Institute, DHEW, and by an institutional grant from the United Foundation of Greater Detroit.