Purification of cytochrome P-450, NADPH-cytochrome P-450 reductase, and epoxide hydratase from a single preparation of rat liver microsomes☆
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2002, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Purification of liver microsomal cytochrome P-450 system. Cytochrome P-450 and NADPH-cytochrome c/P-450 reductase were solubilized and purified following the method of Guengerich and Martin [14]. Cytochrome P-450 [15], NADPH-cytochrome c reductase [16], and protein [17] were assayed as reported earlier.
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This research was supported in part by USPHS Grants ES 00267, ES 01590, and ES 02205. F.P.G. is the recipient of a USPHS Research Career Development Award (ES 00041).