Comparison of the nuclear and cytosolic forms of the Ah receptor from Hepa 1c1c7 cells: Charge heterogeneity and ATP binding properties☆
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Cited by (40)
PAS Proteins: Comparative Biology and Proteasomal Degradation
2010, Comprehensive Toxicology: Second EditionRole of the Per/Arnt/Sim domains in ligand-dependent transformation of the aryl hydrocarbon receptor
2008, Journal of Biological ChemistryCitation Excerpt :Although we hypothesized that following ligand binding Hsp90 association is weakened with the wtAhR (or the PASA-PASB fragment), a higher stringency washing buffer did not result in ligand-dependent differences in binding in the absence of Arnt (data not shown). It is unlikely that Hsp90 fully dissociates from the AhR simply as a result of ligand binding and this is consistent with previous findings that Hsp90 remained bound to the AhR complex through ligand-induced nuclear translocation, and that the ligand-bound nuclear AhR complex contained Hsp90 (8, 28). It is possible that ligand binding results in weakened AhR/Hsp90 interactions, however, this remains to be confirmed.
The mouse and human Ah receptor differ in recognition of LXXLL motifs
2008, Archives of Biochemistry and BiophysicsThe aryl hydrocarbon receptor (AhR) tyrosine 9, a residue that is essential for AhR DNA binding activity, is not a phosphoresidue but augments AhR phosphorylation
2004, Journal of Biological ChemistryCitation Excerpt :These data are consistent with the suggestion that it is not just the negative charge of a putatively phosphorylated tyrosine 9 that is critical for DNA binding. The AhR isolated from cells or synthesized in vitro has been shown to exhibit charge heterogeneity that is consistent with phosphorylation as determined by two-dimensional gel electrophoresis (13, 27, 28). Cytosolic 125I-dioxin-labeled AhR exhibited an apparent isoelectric point (pI) range of 5.2–5.7, while the apparent pI nuclear AhR ranged from 5.5 to 6.2 (28).
Defining the role for XAP2 in stabilization of the dioxin receptor
2003, Journal of Biological Chemistry
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This work was supported in part by National Institute of Environmental Health Science Grant ES-04869. This is technical paper No. 12,910 Indiana Agricultural Experiment Station.
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