Properties of immunoaffinity purified 106-kDa Ca2+ release channels from the skeletal sarcoplasmic reticulum

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Abstract

The sulfhydryl-gated 106-kDa Ca2+-release channel (SG-106) was purified by biotin-avidin chromatography from skeletal sarcoplasmic reticulum (SR) vesicles and used as an antigen to raise polyclonal anti-bodies. Western blots showed that the antisera crossreacted with the antigenic SG-106 and not with SR Ca2+, Mg2+-ATPase or with junctional foot proteins (JFPs) (Zaidi et al., 1989, J. Biol. Chem. 264(36), 21,725–21,736; 21,737–21,747). Polyclonal anti-body-affinity columns were used to selectively purify SG-106-kDa proteins which, upon incorporation in planar bilayers, revealed the presence of a cationic channels with properties similar to “native” Ca2+-release channels obtained through the fusion of SR vesicles with planar bilayers. In agreement with measurements of Ca2+ release from SR vesicles, sulfhydryl oxidizing and reducing agents (i.e., 2,2′-dithiodipyridine and dithiothreitol) respectively increased and decreased the open-time probability of 106-kDa Ca2+-release channels. In contrast with reports on JFPs, ryanodine at 0.5–1 nm increased the open-time probability and at 2–10 nm locked 106-kDa Ca2+-release channels in a closed state rather than an open subconductance state. The SG-106 was activated by millimolar ATP, inhibited by millimolar Mg2+, and blocked by micromolar ruthenium red. Adriamycin (2–10 μm) caused a transient activation of SG-106 Ca2+-release channels, followed by closure in about 5 min, and intermittent activation to a subconductance state. Polyclonal antibodies used to purify the SG-106 also activated the channel when added to the cis side but not the trans side of the bilayer. Thus, SG-106 channels possess features that are similar to “native” SR Ca2+-release channels, are immunologically distinct from JFPs, and interact in seconds with nanomolar ryanodine in planar bilayers.

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      Ca2+-release channels were reconstituted through the fusion of heavy SR vesicles. SR 0 (.5–1 μg protein/ml) was added to the cis side under an osmotic gradient to drive vesicle fusion with a planar lipid bilayer composed of phosphatidylethanolamine:phosphatidylserine:phosphatidylcholine at a ratio of 5:3:2, as described previously (9, 13, 24). The lipids were dissolved in decane at a total concentration of 50 mg/ml, and planar bilayers were formed across a 200-μm diameter hole in a Kynar cup.

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    This work was supported by American Heart Association Grant 87-1065, the Western Pennsylvania Affiliate of the American Heart Association, and National Science Foundation Grant DCB-89 18672 to G. Salama.

    2

    Supported by Physician Investigator Training Grant 5T32-DK07458.

    3

    Recipient of the George Meany Muscular Dystrophy Association Postdoctoral Fellowship.

    4

    Recipient of a postdoctoral fellowship from the Western Pennsylvania Affiliate of the American Heart Association.

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