Purification by cibacron blue F3GA dye affinity chromatography and comparison of NAD(P)H:quinone reductase (E.C.1.6.99.2) from rat liver cytosol and microsomes
References (21)
- et al.
Biochim. Biophys. Acta
(1962) - et al.
Biochem. Pharmacol
(1974) - et al.
Biochem. Biophys. Res. Commun
(1976) - et al.
Arch. Biochem. Biophys
(1978) - et al.
Arch. Biochem. Biophys
(1982) Anal. Biochem
(1976)- et al.
J. Biol. Chem
(1982) - et al.
Arch. Biochem. and Biophys
(1981) - et al.
J. Biol. Chem
(1986) - et al.
J. Biol. Chem
(1974)
Cited by (34)
A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation
2021, Redox BiologyCitation Excerpt :Immunoprecipitation of purified recombinant human NQO1 was carried out as described previously [15]. Recombinant human NQO1 (rhNQO1) was purified as described previously [26]. Reactions (30 μl) containing rhNQO1 (2.5 μg) were incubated in 62.5 mM Tris-HCl, pH 7.0 containing 1.2 M sucrose, 0.01% bromophenol blue and 5 μM FAD in the presence and absence of 500 μM NADH and/or 50 μM MI2321 for 30 min at room temperature.
ATP independent proteasomal degradation of NQO1 in BL cell lines
2012, BiochimieCitation Excerpt :The NQO1*1 protein was purified from cell lines with homozygous wt genotype: K562 and U937 cell lines. Purification gave a better yield using U937 cell line and was performed as described [31]. NQO1 activity was determined by spectrophotometric assay based on the reduction of menadione/cytochrome C [29].
An approach to evaluate two-electron reduction of 9,10-phenanthraquinone and redox activity of the hydroquinone associated with oxidative stress
2007, Free Radical Biology and MedicineThe Nrf2 transcription factor contributes to the induction of alpha-class GST isoenzymes in liver of acute cadmium or manganese intoxicated rats: Comparison with the toxic effect on NAD(P)H:quinone reductase
2007, ToxicologyCitation Excerpt :Cytosolic protein, 50 μg. The enzyme activity was measured as the rate of menadione reduction, which was monitored at 340 nm using a Beckman DU-640 spectrophotometer (Sharkis and Swenson, 1989). The level of alpha- and pi-class GST proteins was quantitatively assayed using a commercial EIA reagent kit with anti-rat GST alpha or anti-rat GST pi class specific antibody and horseradish peroxidase complex (Biotrin, Dublin, Ireland).
Functions and distribution of NQO1 in human bone marrow: Potential clues to benzene toxicity
2005, Chemico-Biological InteractionsCitation Excerpt :Induction is considered to occur via both ARE and XRE elements in the NQO1 promoter [11,12] and is also under the control of the Nrf2/Keap1 system [13,14]. The enzyme is mainly cytosolic although small mitochondrial and microsomal pools have been identified in rat liver [1,15,16]. A small nuclear pool of NQO1 has been characterized in human tumor cells [17] and in humans, NQO1 activity is found mainly in epithelial and endothelial tissues [18].
Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1
2002, Journal of Biological ChemistryCitation Excerpt :RIPA (protease inhibitor mixture) was obtained from Roche Diagnostics GmbH (Mannheim, Germany). Human recombinant NQO1 standard was purified from Escherichia coli by Cibacron blue affinity chromatography as described previously (11, 20). All other chemicals and reagents used in this study were of the highest purity grade available commercially.