Elsevier

Biochemical Pharmacology

Volume 21, Issue 5, 1 March 1972, Pages 619-624
Biochemical Pharmacology

Active transport of 5-hydroxyindoleacetic acid by the rabbit choroid plexus in vitro: Blockade by probenecid and metabolic inhibitors

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Abstract

When rabbit choroid plexuses were incubated at 37° with Krebs-Ringer solutions of [14C]5-hydroxyindoleacetic acid (5-HIAA), the tissue took up the acid by a process showing all the characteristics of active transport. Uptake against a concentration gradient occurred by a saturable process that was inhibited by several metabolic inhibitors. The transport of 5-HIAA was also inhibited by probenecid and para-aminohippuric acid, known inhibitors of organic acid transport systems in many organs. 5-HIAA was found to bind to homogenates of choroid plexus, but the binding would not account for the bulk of acid accumulation seen in the intact tissue. Moreover, the transport of 5-HIAA in rabbit brain cortex slices was not affected by probenecid.

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