Elsevier

Brain Research

Volume 578, Issues 1–2, 24 April 1992, Pages 275-281
Brain Research

Chloride current induced by alcohols in rat dorsal root ganglion neurons

https://doi.org/10.1016/0006-8993(92)90258-BGet rights and content

Abstract

We have recently demonstrated that ethanol and longer-chain alcohols (n-alcohols) enhance γ-aminobutyric acid (GABA)-induced chloride currents before desensitization takes place. The potencies ofn-alcohols increase with lengthening of the carbon chain. We now report thatn-alcohols induce chloride currents by themselves in rat dorsal root ganglion neurons in primary culture. The whole cell variation of the patch clamp techniques was used to record currents as induced by external application of alcohols and other test compounds. Ethanol,n-butanol n-hexanol andn-octanol induced inward currents with their potencies increasing in that order. The potencies were approximately one order of magnitude less than those to augment GABA-induced currents. The maximum amplitudes of currents induced by the alcohols were less than those produced by GABA. Then-octanol-induced currents were carried largely by chloride ions because the reversal potentials were changed according to the Nernst chloride potentials as the internal chloride concentration was changed. Bicuculline and picrotoxin suppressed then-octanol-induced current, and chlordiazepoxide and pentobarbital augmented then-octanol-induced current. Therefore, the alcohol-induced chloride currents flow through the chloride channels associated with the GABAA receptors. When applied after the GABA-induced current was desensitized to a lower level,n-octanol suppressed rather than augmented the current. Thus,n-alcohols mimic barbiturates in augmenting the GABA-induced currents and in generating chloride currents by themselves. These actions of both agents may play a role in causing anxiolytic, sedative and/or anesthetic effects.

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1

Present address: Laboratory of Marine Botany and Environmental Science, Faculty of Fisheries, Kagoshima University, Kagoshima 890, Japan.

2

Present address: National Hospital Shou-rai-soh, 2815 Koizumicho, Yamato-Kooriyama, Nara 639-11, Japan.

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