Fluorescence microscopy of phospholipid monolayer phase transitions

doi:10.1016/0009-3084(91)90078-P

Chemistry and Physics of Lipids

Volume 57, Issues 2–3, March 1991, Pages 227-239

https://doi.org/10.1016/0009-3084(91)90078-P Get rights and content

Abstract

Over many years, a detailed picture of the phase transitions in phospholipid monolayers at the air-water interface has been constructed from extensive studies of the force-area, viscoelastic and surface potential properties of phospholipid monolayers, yet the microscopic nature of the transitions has remaned obscure. Recent investigations have focused specifically on these aspects. Through the use of fluorescence microscopy, electron diffraction and X-ray scattering experiments, in combination with data obtained by classical methods, a wealth of new information regarding the properties of monolayers undergoing phase transistions has been genrated. Direct observation of fluid-solid phase coexistence at the air-water interface has been achieved with fluorescence microscopy and on solid supports with electron miscroscopy. The fluid-solid coexistence region has been studied most thoroughly to date, but regions of gas-fluid and fluid-fluid phase coexistence have also been detected. Numerous factors govern the properties of the coexistence region; however, the promiment features can be explained in terms of a competition between forces: long-range electrostatic forces and short-range attractive forces. In this review these recent experimental findings and theoretical interpretations are summarized.

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Fluorescence microscopy of phospholipid monolayer phase transitions

Abstract

Biochim. Biophys. Acta

J. Colloid Interface Sci.

Biophys. J.

Thin Solid Films

Thin Solid Films

J. Colloid Interface Sci.

Biophys. J.

Physica A

Biophys. J.

Biophys. J.

Biochim. Biophys. Acta

J. Colloid Interface Sci.

Thin Solid Films

Biochim. Biophys. Acta

Thin Solid Films

Physica A

J. Mol. Struct.

Thin Solid Films

Thin Solid Films

Biochim. Biophys. Acta

Ben Franklin Stilled the Waters

J. Am. Chem. Soc.

J. Chem. Phys.

The Physics and Chemistry of Surfaces

The Physical Chemistry of Surface Films

Interfacial Phenomena

Insoluble Monolayers at the Liquid-Gas Interface

Physical Chemistry of Surfaces

J. Exp. Med.

J. Pharm. Pharmacol.

Koll. Zietschr. Zeitschr. Polym.

J. Phys.

Langmuir

Lipid and Biopolymer Monolayers at Liquid Interfaces

The Physics and Chemistry of Surfaces

Ber. Bunsenges. Chem.

Phys. Rev. Lett.

J. Phys. Chem.

Biochemistry

J. Phys. Chem.

J. Phys. Chem.

Rev. Sci. Instr.