Expression of liver phenotypes in cultured mouse hepatoma cells: Synthesis and secretion of serum albumin

doi:10.1016/0012-1606(73)90008-0

Developmental Biology

Volume 35, Issue 1, November 1973, Pages 83-96

https://doi.org/10.1016/0012-1606(73)90008-0 Get rights and content

Abstract

A permanent cell line, designated Hepa, has been isolated from a mouse hepatoma, BW 7756. The cell line synthesizes and secretes albumin at rates appreciably higher than previously reported hepatomas adapted to in vitro conditions. Monospecific antimouse serum albumin was produced in rabbits, and mouse serum albumin secreted by the hepatoma cells was identified by double diffusion, immunoelectrophoresis, and radioimmunodiffusion. A quantitative immunoassay was used to measure albumin secretion and to study the effects of culture conditions on albumin secretion. A subclonal analysis was performed to study the homogeneity and stability of cloned hepatoma lines in respect to albumin secretion. Different secretion rates were observed during the culture cycle. Significant clonal variation in respect to albumin secretion was found among ten subclones.

The significance of clonal variation is discussed in relation to the study of epigenetic control of albumin expression in somatic hybrid cells.

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^☆: This study was supported by NIH grant USPHS 5-R01-BM 09966 and NSF grant GB 34303, and a postdoctoral fellowship from the Swiss National Science Foundation (awarded to H.P.B.).

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Full paperExpression of liver phenotypes in cultured mouse hepatoma cells: Synthesis and secretion of serum albumin☆

Abstract

Biochem. Biophys. Res. Commun

Anal. Biochem

J. Biol. Chem

J. Biol. Chem

Variation in tyrosine aminotransferase induction in HTC cell clones

Science

Production of antiserum

Stimulation of aryl hydrocarbon hydroxylase activity in established cell lines derived from rat or mouse hepatoma or from normal rat liver

Biochim. Biophys. Acta

Hepa-1: A cell line expressing liver functions in vitro

Expression of differentiated functions in hepatoma cell hybrids. II. Aldolase

J. Cell. Physiol

Morphology and function of cells of human embryonic liver in monolayer culture

J. Cell Biol

Neoplastic transformation in vitro of a clone of adult liver epithelial cells into differentiated hepatoma-like cells under conditions of nutritional stress

Studies on the synthesis of serum albumin by the isolated microsome fraction from rat liver

Biochem. J

Clonal culture of differentiated cells from mammals: Rat liver cell culture

Carnegie Inst. Wash. Yearb

A murine cell line expressing liver functions in vitro

Activation of a human differentiated phenotype (albumin synthesis) in mouse hepatoma × human leucocyte somatic cell hybrids

Regulation of gene expression in somatic cell hybrids: a review

In Vitro

Hybridization of Somatic Cells

Evidence for the participation of the golgi apparatus in the intracellular transport of nascent albumin in the liver cell

J. Cell Biol

Methods for measuring rates of synthesis of albumin by the isolated perfused rat liver

Biochem. J

The localization of albumin and fibrinogen in human liver cells

J. Cell Biol

Full paper
Expression of liver phenotypes in cultured mouse hepatoma cells: Synthesis and secretion of serum albumin☆