Potassium concentration and number of neurons in cultures of dissociated ganglia

doi:10.1016/0014-4886(70)90197-4

Experimental Neurology

Volume 27, Issue 1, April 1970, Pages 16-22

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Abstract

A new quantitative method is described for investigating the effect of the physical and chemical environment on neuron survival in long-term culture. Dissociated nervous tissue was cultured in media containing potassium concentration ranging from 0.8 to 80.8 mm. After 11 days in culture approximately ten times as many neurons were present for 40.8 mm K+ medium as compared to the 6.8 K+ medium.

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¹: Supported in part by funds from the National Research Council of Canada. We thank Mr. George Healy and Dr. R. C. Parker of Connaught Medical Laboratories for providing potassium and sodium free CMRL-1415. We also thank Messrs M. Jones, M. Cosmon, and Ping Lim for their technical assistance.

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Potassium concentration and number of neurons in cultures of dissociated ganglia

Abstract

Arch. Biochem. Biophys.

Int. Rev. Cytol.

Develop. Biol.

Exp. Neurol.

Exp. Cell Res.

Reconstituted rat tail collagen used as a substrate for tissue cultures on cover slips

Lab. Invest.

Effect of supernatant K level on cardiac activity in qantitative tissue culture

Nature

Role of potassium in physiological processes

Physiol. Rev.

Observations on living developing nerve fibres