[5] Terminal transferase: Use in the tailing of DNA and for in Vitro mutagenesis
References (31)
J. Biol. Chem.
(1960)- et al.
Biochim. Biophys. Acta
(1962) - et al.
J. Mol. Biol.
(1973) - et al.
Cell
(1977) - et al.
J. Biol. Chem.
(1967) - et al.
Gene
(1978) Gene
(1978)Gene
(1981)- et al.
Biochim. Biophys. Acta
(1981) - et al.
J. Biol. Chem.
(1976)
Proc. Natl. Acad. Sci. U.S.A.
Nature (London)
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A Mechanism to Minimize Errors during Non-homologous End Joining
2020, Molecular CellCitation Excerpt :Of the ends tested in this study, such a substrate had the most variable junctions (Figure 1B, 6), although this variability was still largely limited to one- to four-nucleotide insertions mediated by pol μ. Second, V(D)J recombination uses the lymphocyte-specific terminal deoxynucleotidyl transferase (TdT), which has robust template-independent synthesis activity on 3′ overhangs (Deng and Wu, 1983). Signal ends (Figure S7C), which are blunt and therefore do not require Artemis activity and do not present the preferred TdT substrate, are typically joined without any insertions or deletions (Gellert, 2002), similar to compatible spontaneous DSBs (Figure 1B, 1–3).
Studies on genomic DNA topology and stability in brain regions of Parkinson's disease
2006, Archives of Biochemistry and BiophysicsCitation Excerpt :The incorporation of the 3[H]-dTTP into DNA would be proportional to the number of double strand breaks (DSBs) in the DNA. From the conditions of incubation [46,47], it is assumed that about 50 TMP residues are added at each of the 3′-ends of the duplex DNA. From this, it is calculated that each femtomole of TMP incorporation would be equivalent to 1.2 × 107 3′-ends or half of that number minus one DSBs.
Preparation of Labeled DNA, RNA, and Oligonucleotide Probes
2022, Cold Spring Harbor Protocols