[34] Preparation of recombinant ADP-ribosylation factor
References (28)
- et al.
J. Biol. Chem.
(1982) - et al.
J. Biol. Chem.
(1984) - et al.
J. Biol. Chem.
(1986) - et al.
J. Biol. Chem.
(1991) - et al.
Cell
(1991) - et al.
J. Biol. Chem.
(1992) - et al.
J. Biol. Chem.
(1992) - et al.
J. Mol. Biol.
(1986) - et al.
J. Biol. Chem.
(1989) - et al.
J. Biol. Chem.
(1982)
Gene
Mol. Cell. Biol.
Cited by (65)
ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion
2008, Journal of Biological ChemistryThe effect of missense mutations in the RhoGAP-homology domain on ocrl1 function
2006, Molecular Genetics and MetabolismAssays and properties of the Arf GAPs AGAP1, ASAP1, and Arf GAP1
2005, Methods in EnzymologyCitation Excerpt :Either myristoylated Arf1 or [L8K]Arf1 can be used to achieve more efficient exchange and higher concentrations of Arf1•GTP when necessary for saturation kinetics. When loading with a radiolabeled GTP, myristoylated Arf1, or [L8K]Arf1 at concentrations up to 200 μM is incubated in buffer B with up to 400 μM [α32P]GTP or [γ32P]GTP (SA = 10,000–50,000 cpm/pmol) (GTP at a 2 to 5 fold excess of Arf1) and either 3 mM dimyristoylphosphatidylcholine/0.1% (w/v) sodium cholate, pH 7.4, (described in this series, Randazzo et al., 1992) or 500 μM phospholipids in the form of LUVs for 30–60 min at 30°. MgCl2 is then added to adjust the free Mg2+ concentration to 1 mM and the reaction mixture is chilled to 4° until use.
The role of ADP-ribosylation factor and SAR1 in vesicular trafficking in plants
2004, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :This key biochemical feature allows Arf to couple the GTP–GDP conformational switch to membrane binding [63]. Arf has weak structural homology with other ras-like GTP binding proteins and has specific sequence motifs, e.g., D(V/I)GGQ instead of DTAGQ as found in the other subfamilies at the second consensus GTP-binding domain [64]. Six Arf proteins have been identified in mammals, three in yeast, 12 in A. thaliana[28,65,66], and several Arf-related proteins have been found in mammals, yeast and plants [66,67].