The ability of a ternary complex to form over the serum response element correlates with serum inducibility of the human c-fos promoter

doi:10.1016/0092-8674(89)90579-5

Cell

Volume 56, Issue 4, 24 February 1989, Pages 563-572

https://doi.org/10.1016/0092-8674(89)90579-5 Get rights and content

Abstract

Rapid induction of c-fos transcription by serum and phorbol esters requires the serum response element (SRE). The SRE contains a 20 bp element of interrupted dyad symmetry (DSE) that is bound by p67/SRF. We have identified a hitherto unrecognized protein with an apparent size of 62 kd. This novel component, p62, is shown to be an integral but physically separable part of a ternary complex formed with p67/SRF and the SRE. Alone, p62 fails to bind the SRE but requires DSE-bound p67/SRF and sequences both within and outside the DSE for its interaction with DNA. In vivo, the response of the c-fos promoter to serum is severely impaired by mutations that abolish ternary complex formation in vitro.

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Cell

ArticleThe ability of a ternary complex to form over the serum response element correlates with serum inducibility of the human c-fos promoter

Abstract

Cell

Cell

Anal. Biochem.

J. Mol. Biol.

Meth. Enzymol.

Cell

Cell

Cell

Virology

Cell

Cell

Cell

Complexity of the early genetic response to growth factors in mouse fibroblasts

Mol. Cell. Biol.

Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis

Science

Activation of the c-fos gene by UV and phorbol ester: different signal transduction pathways converge to the same enhancer element

Oncogene

The structure and function of the fos proto-oncogene

CRC Crit. Rev. Biochem.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

Nucl. Acids Res.

Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis

Nucl. Acids Res.

A gel electrophoresis method for quantifying the binding of protein to specific DNA regions: applications to components of the E. coli lactose operon regulatory system

Nucl. Acids Res.

The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP

Genes Dev.

Article
The ability of a ternary complex to form over the serum response element correlates with serum inducibility of the human c-fos promoter