PDGF β-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex

doi:10.1016/0092-8674(90)90220-9

Cell

Volume 61, Issue 1, 6 April 1990, Pages 125-133

https://doi.org/10.1016/0092-8674(90)90220-9 Get rights and content

Abstract

Platelet-derived growth factor (PDGF) stimulated the tyrosine phosphorylation of the GTPase activating protein (GAP) in 3T3 cells and in CHO cells expressing wild-type PDGF receptors, but not in several CHO cell lines expressing mutant receptors defective in transmitting mitogenic signals. Following PDGF treatment of cells, GAP physically associated with the PDGF receptor and with Raf-1, phospholipase c-γ, and Pl-3 kinase, suggesting that PDGF induced the formation of complexes of signaling molecules. The association of GAP with the PDGF receptor and the phosphorylation of GAP were reconstituted in vitro using purified protein and in insect cells expressing murine PDGF receptor and human GAP. However, in cells transformed by activated c-Ha-ras, which are defective in certain responses to PDGF, GAP failed to associate with the PDGF receptor or increase its phosphotyrosine content in response to PDGF. The association of GAP with ligand-activated PDGF receptors may directly link PDGF and ras signaling pathways.

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ArticlePDGF β-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex

Abstract

Cell

J. Biol. Chem.

Cell

Cell

Cell

J. Biol. Chem.

J. Biol. Chem.

Cell

Cell

Cell

Cell

Cell

J. Biol. Chem.

Cell

Loss of platelet-derived growth factor-stimulated phospholipase activity in NIH-3T3 cells expressing the EJ-ras oncogene

Voltage-sensitive calcium channels in normal and transformed 3T3 fibroblasts

Science

Role of phosphatidylinositol kinase in PDGF receptor signal transduction

Science

Phosphorylation of GAP and GAP-associated proteins by transforming and mitogenic tyrosine kinases

Nature

A PDGF receptor domain essential for mitogenesis but not for many other responses to PDGF

Nature

Role of tyrosine kinase and membrane-spanning domains in signal transduction by the platelet-derived growth factor receptor

Mol. Cell. Biol.

Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family

J. Virol.

Article
PDGF β-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex