Elsevier

Peptides

Volume 12, Issue 1, January–February 1991, Pages 167-176
Peptides

Article
Visualization of cholecystokinin receptors on a subset of human monocytes and in rat spleen

https://doi.org/10.1016/0196-9781(91)90184-QGet rights and content

Abstract

Direct radioreceptor binding experiments and Scatchard analysis reveal CCK receptors on elutriator purified human peripheral blood monocytes, but not on purified human T cells. The monocyte receptors have a single class of high (0.1 nM) affinity binding sites. A structure-function analysis of monocyte binding by different CCK analogs correlates well with previously demonstrated chemotactic responses in monocytes and receptors in brain tissue. Biochemical cross-linking indicates that the monocyte CCK recognition molecule is comparable in molecular size to that in brain membranes. Utilizing a novel fluoresceinated Texas Red-CCK conjugate we have visualized that up to 20% of human peripheral monocytes bear receptors for CCK. A discrete and anatomically significant distribution of CCK receptors in rat spleen is shown by film autoradiography of tissue sections. A more detailed microscopic analysis identifies a dendritic population of monocyte-derived cells within the periarteriolar lymphocyte sheath (PALS) of the white pulp as the CCK receptor-bearing cell in spleen. The anatomical localization of receptor-bearing cells within the PALS region suggests a role for CCK in the antigen processing and sensitization phases of the immune response via regulatory effects of this peptide on a specific, local macrophage-related cell population.

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      CCK2 receptors are predominantly located throughout the brain, CNS and in specific parts of the gastrointestinal tract. They also are present on some lymphocytes and splenocytes (e.g. as monocytes, T-cells, and lymphoblasts [31–33,39,43,44].1 The simplest mechanistic pathway that can mediate the binding of, for example, a peptide hormone (such as adrenaline and melatonin) to transmembrane receptors involves a lock-and-key mechanism, where the hormone binds directly to the transmembrane receptor from the extracellular fluid, triggering a cellular response via a secondary messenger pathway [31–33,39].

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    1

    Present address: Department of Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy.

    2

    Present address: Department of Internal Medicine, University of Innsbruck, A-6020 Innsbruck, Austria.

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