Detection by Northern analysis of α1-adrenergic receptor gene transcripts in the rat

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Abstract

In Northern blots of total cellular and poly(A+) RNA isolated from rat liver, renal cortex, spleen, and brain probed with a full-length cDNA encoding the hamster α1-adrenergic receptor, hybridization was observed to two distinct mRNAs, at ~3.3 kb and ~2.7 kb. Only the ~2.7 kb mRNA species was visualized in Northern blots of total cellular and poly(A+) RNA isolated from cardiac ventricular muscle. From screening a rat heart cDNA library with the full-length hamster α1-adrenergic receptor cDNA, a 632 base pair cDNA was isolated. Based upon its high degree of identity, 86% at the nucleotide level, with the hamster α1-adrenergic receptor cDNA, this cDNA was considered to include the 3' end of the rat α1-adrenergic receptor. When used as a probe in Northern blots of liver RNA, both the ~ 3.3 kb and ~ 2.7 kb mRNAs were visualized. Both mRNA species were expressed in fetal as well as adult liver, but steady-state levels of each gene transcript were ~ 3-fold higher in adult compared to fetal liver. Finally, results from Southern analysis of restriction enzyme fragments of genomic DNA suggest that the two gene transcripts may be products of a single gene.

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    Present address: Laboratory of Molecular Endocrinology, Massachusetts General Hospital, 50 Blossom Street, Boston, MA 02114, U.S.A.

    ∗∗

    Present address: Department of Anatomy, University of Arkansas for Medical Sciences, 4301 West Markham Street, Little Rock, AR 72205-7199, U.S.A.

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