Elsevier

Neuroscience Letters

Volume 158, Issue 1, 6 August 1993, Pages 44-46
Neuroscience Letters

Normalization of tyrosine hydroxylase activity in vivo in the striatum of transgenic mice carrying human tyrosine hydroxylase gene: a microdialysis study

https://doi.org/10.1016/0304-3940(93)90608-NGet rights and content

Abstract

Using a microdialysis method, we observed a similar steady-state l-3,4-dihydroxyphenylalanine accumulation in the striatum of transgenic mice carrying the human tyrosine hydroxyIase (TH) gene after NSD-1015 perfusion (10−4 M) as compared to nontransgenic mice. Basal extracellular levels of 3,4-dihydroxyphenylacetic acid before the perfusion of NSD-1015 were also comparable in both transgenic and non-transgenic striata. The results suggest that the in vivo activity of TH in the striatum of transgenic mice was retained to the normal level by some regulatory mechanism(s) in spite of the increased expression of the enzyme protein.

References (8)

  • J.W. Brock et al.

    Effect of chronic cocaine on dopamine synthesis in the nucleus accumbens as determined by microdialysis perfusion with NSD-1015

    Neurosci. Lett.

    (1990)
  • N. Kaneda et al.

    Tissue-specific and high-level expression of the human tyrosine hydroxylase gene in transgenic mice

    Neuron

    (1991)
  • K. Kiuchi et al.

    Regulatory mechanism of dopamine biosynthesis in the striatum of transgenic mice carrying human tyrosine hydroxylase gene

    Neurosci. Lett.

    (1993)
  • Y. Liu et al.

    A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter

    Cell

    (1992)
There are more references available in the full text version of this article.

Cited by (15)

  • Chapter 5.1 Microdialysis in genetically altered animals

    2006, Handbook of Behavioral Neuroscience
    Citation Excerpt :

    As one of the most informative and reliable tool to measure extracellular neurotransmitter concentrations, microdialysis has shown great utility in characterization of such models. The first reports describing application of microdialysis technique to genetically altered animals are dated as early as 1993 and 1994 (Nakahara et al., 1993; Nagatsu et al., 1994), when techniques to develop transgenic mice were in infancy. These investigations give ambiguous results, however, most likely due to limitations of these early genetic approaches used to overexpress proteins of interest.

  • Microdialysis in mice for drug delivery research

    2000, Advanced Drug Delivery Reviews
View all citing articles on Scopus
View full text