Journal of Chromatography B: Biomedical Sciences and Applications
Short communicationHigh-performance liquid chromatographic quantification of 4-methylumbelliferyl-β-d-glucuronide as a probe for human β-glucuronidase activity in tissue homogenates
References (14)
- et al.
J. Pediatr.
(1973) - et al.
Clin. Chim. Acta
(1992) - et al.
Clin. Chim. Acta
(1991) - et al.
Biochem. Pharmacol.
(1993) - et al.
Clin. Chim. Acta
(1967) - et al.
J. Biol. Chem.
(1951) - et al.
Pediatr. Res.
(1993)
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