Hydrogen peroxide production by red blood cells

doi:10.1016/0891-5849(94)90249-6

Free Radical Biology and Medicine

Volume 16, Issue 1, January 1994, Pages 123-129

https://doi.org/10.1016/0891-5849(94)90249-6 Get rights and content

Abstract

Red blood cells are frequently employed in studies of oxidative stress. Technical difficulties have previously prevented the measurement of H₂O₂ production by red blood cells, except during exposure to certain drugs or toxicants. We now show that a combination of glutathione depletion and 3-amino-1,2,4-triazole (aminotriazole) treatment can be used to measure the endogenous generation of H₂O₂ by red blood cells. In our studies, aminotriazole was used as an H₂O₂ dependent (irreversible) catalase inhibitor, and catalase inhibition was used as an indirect measure of H₂O₂ production. Our results indicate that H₂O₂ is generated at a rate of 1.36 ± 0.2 μM/h (3.9 ± 0.6 nmol·h⁻¹·gHb⁻¹), and that the steady-state red blood cell concentration of H₂O₂ is approximately 2 × 10⁻¹⁰M. Kinetic comparisons of H₂O₂ production and oxyhemoglobin autooxidation (which generates O₂^.− that dismutes to H₂O₂) indicate that the latter is probably the main source of H₂O₂ in red blood cells.

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^☆: This research was supported by grant No. ES-03598 from the NIH/NIEHS to K.J.A. Davies C. Giulivi was the recipient of a fellowship from Fundacion Antorchas (Buenos Aires, Argentina).

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Brief communicationHydrogen peroxide production by red blood cells☆

Abstract

Biochim. Biophys. Acta

Arch. Biochem. Biophys.

Meth. Enzymol.

J. Biol. Chem.

Meth. Enzymol.

Blood

J. Biol. Chem.

Arch. Biochem. Biophys.

Biochim. Biophys. Acta

Clin. Chim. Acta

J. Biol. Chem.

Biochim. Biophys. Acta

J. Biol. Chem.

FEBS Lett.

J. Biol. Chem.

In vivo generation of H2O2 in mouse erythrocytes by hemolytic agents

J. Pharmac. Exp. Ther.

In vivo generation of hydrogen peroxide from 6-hydroxy dopamine

Experientia

Irreversible reaction of 3-amino-1,2,4-triazole and related inhibitors with the protein of catalase

Biochem. J.

Studies on the mechanism of inhibition of liver and erythrocyte catalase activity by 3-amino-1,2,4-triazole (AT)

J. Pharmac. Exp. Ther.

Hydrogen peroxide production by rat brain in vivo

J. Neurochem.

Brief communication
Hydrogen peroxide production by red blood cells☆

In vivo generation of H₂O₂ in mouse erythrocytes by hemolytic agents