Primary structure and expression of β2: A novel subunit of neuronal nicotinic acetylcholine receptors

doi:10.1016/0896-6273(88)90208-5

Neuron

Volume 1, Issue 1, March 1988, Pages 45-54

https://doi.org/10.1016/0896-6273(88)90208-5 Get rights and content

Abstract

A new subunit, β2, of the neuronal nicotinic receptor family has been identified. This subunit has the structural features of a non-agonist-binding subunit. We provide evidence that β2 can substitute for the muscle β1 subunit to form a functional nicotinic receptor in Xenopus oocytes. Expression studies performed in oocytes have demonstrated that three different neuronal nicotinic acetylcholine receptors can be formed by the pairwise injection of β2 mRNA and each of the neuronal α subunit mRNAs. The β2 gene is expressed in PC12 cells and in areas of the central nervous system where the α2, α3, and α4 genes are expressed. These results lead us to propose that the nervous system expresses diverse forms of neuronal nicotinic acetylcholine receptors by combining β2 subunits with different agonist-binding α subunits.

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Neuron

Primary structure and expression of β2: A novel subunit of neuronal nicotinic acetylcholine receptors

Abstract

Neurosci. Lett.

Plasmid

Cell

Gene

J. Biol. Chem.

Brain Res.

J. Mol. Biol.

J. Biol. Chem.

Biochem. Biophys. Res. Common.

FEBS Lett.

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose

Isolation of a clone coding for the α-subunit of a mouse acetylcholine receptor

J. Neurosci.

Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor a-subunit

Nature

Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family

Proc. Natl. Acad. Sci. USA

A method for isolation of intact, translationally active ribonucleic acid

DNA

Nicotinic binding in the rat brain: autoradiographic comparison of [3H]-acetylcholine, [3H]-nicotine, and [125l]-α-bungaro-toxin

J. Neurosci.

Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor γ subunit

Brain and muscle nicotinic acetylcholine receptors are different but homologous proteins

Mapping of brain areas expressing RNA homologous to two different acetylcholine receptor α-subunits cDNAs

Nicotinic binding in the rat brain: autoradiographic comparison of [³H]-acetylcholine, [³H]-nicotine, and [¹²⁵l]-α-bungaro-toxin