Modulation of excitatory synaptic transmission by drugs that reduce desensitization at AMPA/kainate receptors

doi:10.1016/0896-6273(91)90342-W

Neuron

Volume 7, Issue 6, December 1991, Pages 971-984

https://doi.org/10.1016/0896-6273(91)90342-W Get rights and content

Abstract

Desensitization at AMPA/kainate receptors has been proposed to contribute to the decay of excitatory synaptic currents. We examined the action of aniracetam, wheat germ agglutinin (WGA), and concanavalin A (Con A), drugs that act via separate mechanisms to reduce desensitization evoked by l-glutamate in rat hippocampal neurons. The decay of excitatory synaptic currents, and sucrose-evoked miniature excitatory postsynaptic currents (EPSCs) was slowed 2- to 3-fold by aniracetam. In contrast, WGA increased the EPSC decay time constant only 1.3-fold and Con A had no effect. Aniracetam increased the magnitude of stimulus-evoked EPSCs 1.9-fold; variance analysis suggests a postsynaptic mechanism of action. WGA and Con A reduced EPSC amplitude via a presynaptic mechanism. Aniracetam increased the burst length of l-glutamate-activated single-channel responses. Simulations suggest that aniracetam either slows entry into a desensitized state or decreases the closing rate constant for ion channel gating.

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^‡: L. V. and D. K. P. contributed equally to this study.

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Neuron

ArticleModulation of excitatory synaptic transmission by drugs that reduce desensitization at AMPA/kainate receptors

Abstract

J. Neurosci. Meth.

Biophys. J.

Neuron

Neurosci. Lett.

Neuron

Neuron

Brain Res.

Neuron

Biophys. J.

Neuron

Voltage-dependent gating of single sodium channels from mammalian neuroblastoma cells

J. Neurosci.

A reinterpretation of mammalian sodium channel gating based on single channel recording

Nature

NMDA and non-NMDA receptors are co-localized at individual excitatory synapses in cultured rat hippocampus

Nature

Origin of variability in quantal size in cultured hippocampal neurons and hippocampal slices

Molecular cloning and functional expression of glutamate receptor genes

Science

Fast events in singlechannel currents activated by acetylcholine and its analogues at the frog muscle end-plate

J. Physiol.

The relationship between the mode of operation and the dimensions of the functional regions at synapses and motor end-organs

Effects of the lectin concanavalin-A on the regulation of second messengers and parathyroid hormone release by extracellular Cal' in bovine parathyroid cells

Endocrinology

Presynaptic glutamate receptors depress excitatory monosynaptic transmission between mouse hippocampal neurones

J. Physiol.

Article
Modulation of excitatory synaptic transmission by drugs that reduce desensitization at AMPA/kainate receptors