Changes in competence determine the timing of two sequential glucocorticoid effects on sympathoadrenal progenitors

doi:10.1016/0896-6273(92)90285-L

Neuron

Volume 8, Issue 3, March 1992, Pages 589-604

https://doi.org/10.1016/0896-6273(92)90285-L Get rights and content

Abstract

We have studied the control of adrenal chromaffin cell development by glucocorticoids (GCs), in a reconstituted in vitro system. The development of the chromaffin phenotype involves two sequential, GC-dependent events: the decision not to become a sympathetic neuron, and the decision to express the epinephrine-synthesizing enzyme, phenylethanolamine-N-methyltransferase (PNMT). Both decisions appear to be mediated by the type II GC receptor. Competence to express PNMT develops on a schedule in vitro which parallels that seen in vivo, but only in progenitors that have first failed to undergo neuronal differentiation. The schedule of PNMT induction is thus controlled by the time of appearance of neither the inducing signal nor its receptor, as previously suggested, but rather by a cell-intrinsic timed process in chromaffin precursors. The two effects of GCs are pharmacologically distinct, suggesting that the GC receptor may interact differently with different genes in the same cell, in a manner that changes with development.

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Neuron

ArticleChanges in competence determine the timing of two sequential glucocorticoid effects on sympathoadrenal progenitors

Abstract

Cell

Cell

Neuron

Dev. Biol.

Dev. Biol.

Neuron

Neuron

Dev. Brain Res.

Neuron

J. Biol. Chem.

Neuron

Int. J. Dev. Neurosci.

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J. Steroid Biochem.

J. Steroid Biochem.

Steroids

Cell

Steroids

J. Mol. Biol.

Cell

Int. J. Dev. Neurosci.

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Dev. Biol.

Neuron

Dev. Biol.

Steroids

Neuroscience

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Cell

A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1)

J. Immunol.

Radioimmunoassayof plasma progesterone

J. Clin. Endocrinol. Metab.

Cell fate and gene expression in the developing neural crest

Article
Changes in competence determine the timing of two sequential glucocorticoid effects on sympathoadrenal progenitors