Original ArticlesSister of P-glycoprotein expression in different tissues
Section snippets
RT-PCR
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. It was prepared from freshly excised porcine brain grey cortex, liver, kidney cortex, small- and large-gut mucosa. RNA from confluent (10 days in culture) porcine brain capillary endothelial cells was also examined. Primary cultures of these endothelial cells were prepared as described earlier [9]. After DNase digestion, RNA was quantified using a GeneQuant photometer (Pharmacia). Its integrity
Results and discussion
Brain grey cortex, liver, kidney, small- and large-gut mucosa are known to express high levels of Pgps 3, 10, 11, 12, 13, 14, 15, 16. The distribution of spgp was therefore investigated in these tissues. With RT-PCR, spgp expression could be demonstrated in brain, liver, small and large gut, whereas no spgp could be detected in the kidney (Fig. 1). The identity of the gene product with spgp was confirmed by cDNA sequence analysis. To determine which types of Pgps are expressed by these porcine
Acknowledgements
This work was supported by the Swiss National Science Foundation (Grant 32-052918.97), a scholarship to M.T. from the Association of Chemical Industries Basel, research grants from the Sandoz Foundation, and the ASTRA Research Funds of the Dept. of Internal Medicine of the University Hospital, Basel. We also thank U. Behrens for excellent technical assistance and I. Arnet for many valuable discussions.
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