Short communicationBinding sites for the plant lectin Bandeiraea simplicifolia I-isolectin B4 are expressed by nociceptive primary sensory neurones
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Acknowledgements
This study was suported by a QUT REA Grant to M.B.P. and an Australian Postgraduate Award to M.B.G.
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Expression of NPR-B in neurons of the dorsal root ganglia of the rat
2013, PeptidesCitation Excerpt :NPR-B immunoreactivity was also found in IB4-positive small neurons. It is known that IB4 is expressed by small DRG neurons, nociceptor neurons, which are involved in transmission of thermal, mechanical, and chemical stimuli [12,13], and BNP is also expressed in IB4-positive neurons [31]. In addition, BNP suppresses the excitability of small DRG neurons and reduces inflammatory pain by activating the NPR-A pathway [31].
Specific functioning of Cav3.2 T-type calcium and TRPV1 channels under different types of STZ-diabetic neuropathy
2013, Biochimica et Biophysica Acta - Molecular Basis of DiseaseCitation Excerpt :Therefore, we checked whether different types of PDN were associated with changes in a proportion of nonpeptidergic versus peptidergic C-type nociceptors. Using isolectin B4 (IB4) [50] for in vitro labeling of nonpeptidergic neurons [34], small DRG neurons (n = 608), considered as C-type nociceptors [51], were divided into three classes based on the intensity of IB4 binding [43]: strongly IB4-positive, weakly IB4-positive and IB4-negative. Fractions of these neuronal classes were unchanged in all PDN groups compared to control (p > 0.05, χ2-test; Fig. 2).
GRK2 in sensory neurons regulates epinephrine-induced signalling and duration of mechanical hyperalgesia
2011, PainCitation Excerpt :Our data show that the percentages of neurons of all sizes do not differ among genotypes. Small-sized primary sensory neurons have been reported to selectively express IB4 binding sites [8], and relatively high IB4 binding is specific for C-nociceptor-type neurons [7]. When analysing the percentage of IB4(+) and IB4(−) DRG neurons in WT and GRK2-deficient mice, we found that there was no difference in the percentage of IB4(+) neurons.
Chloride transporters in presynaptic inhibition, pain and neurogenic inflammation
2010, Physiology and Pathology of chloride transporters and channels in the nervous systemGlial cell line-derived neurotrophic factor acutely modulates the excitability of rat small-diameter trigeminal ganglion neurons innervating facial skin
2010, Brain, Behavior, and ImmunityCitation Excerpt :In addition, the expression of GDNF receptors, and in particular GFRα-1 immunoreactivity, was also seen in small-diameter DRG neurons, and corresponded to those which bind the nociceptive marker, IB4, which is believed to be involved in nociceptive function (Silverman and Kruger, 1988; Akkina et al., 2001; Bennett et al., 1998; Molliver et al., 1997; Leitner et al., 1999). In fact, most IB4 positive nociceptors are sensitive to noxious mechanical stimuli (Gerke and Plenderleith, 2001; Vulchanova et al., 2001). Previous studies indicated that human small- and medium-diameter TRG neurons co-express GDNF and GFRα-1 (Quartu et al., 1999, 2006).
Reg-2 expression in dorsal root ganglion neurons after adjuvant-induced monoarthritis
2008, NeuroscienceCitation Excerpt :Although cytokines are thought to play an important role in inflammation (Marchand et al., 2005), many of their effects on DRG neurons appear to be indirect and mediated by NGF (Woolf et al., 1997). It is clear that many IB4 neurons are nociceptors (Gerke and Plenderleith, 2001) but their exact role is unclear, and studies that have compared the responses properties of IB4-positive and IB4-negative neurons have identified only minor differences (Stucky and Lewin, 1999; Petruska et al., 2000; Dirajlal et al., 2003; Liu et al., 2004; Breese et al., 2005). Recently, however, a role for IB4 cells in inflammation has been proposed based on data showing that TRPV1 expression increases in IB4-positive cells (Amaya et al., 2004; Breese et al., 2005) as well as in IB4-negative (trkA immunoreactive) cells (Amaya et al., 2004) during inflammation.