A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity
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Keywords
Cell-mediated cytotoxicity
Natural killer activity
NK
CTL
AlamarBlue
Fluorescence
Abbreviations
CTL, cytotoxic T lymphocyte
E:T, effector:target ratio
EBV, Epstein Barr virus
FCS, fetal calf serum
MTT, 3-[4-5-Dimethylthiazol-2-yl]-2-5-diphenyl bromide tetrazolium bromide
MUH, 4-methylumbelliferyl heptanoate
NK, natural killer
O/N, overnight
PBMC, peripheral blood mononuclear cells
rIL-2, recombinant Interleukin 2
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