Elsevier

Life Sciences

Volume 60, Issue 10, 31 January 1997, Pages 715-724
Life Sciences

Identification of a membrane-bound carboxypeptidase as the mammalian homolog of duck GP180, a hepatitis B virus-binding protein

https://doi.org/10.1016/S0024-3205(96)00642-XGet rights and content

Abstract

A unique membrane-bound carboxypeptidase was discovered and characterized in membrane fractions of human skin fibroblasts and the mouse monocyte-macrophage cell line J774A.1 and was partially purified from human placenta. Enzymatic characterization identified it as a new member of the regulatory B-type metallocarboxypeptidases, different from carboxypeptidases B, E, M, N and U. It is, however, similar to the newly described bovine carboxypeptidase D, suggested to be a homolog of duck gp180, a 180 kDa hepatitis B virus-binding protein. To prove this, a partial cDNA encoding a 20 kDa fragment of the human homolog of duck gp180 was expressed in bacteria and the recombinant protein was purified. Antibodies raised to the protein immunoprecipitated 94% or 72% of the low pH carboxypeptidase activity in human skin fibroblasts or J774A.1 cells and gave a 175 kDa protein band in Western blots. Thus, carboxypeptidase D is the mammalian homolog of duck gp180 and is distributed in a variety of different cell types.

References (14)

  • R.A. Skidgel

    Trends Pharmacol. Sci.

    (1988)
  • R.A. Skidgel et al.

    J. Biol. Chem.

    (1989)
  • L.D. Fricker et al.

    J. Biol. Chem.

    (1990)
  • R.A. Skidgel et al.

    Biochem. Pharmacol.

    (1984)
  • F. Tan et al.

    Meth. Enzymol.

    (1995)
  • K. Kuroki et al.

    J. Biol. Chem.

    (1995)
  • G.B. McGwire et al.

    J. Biol. Chem.

    (1995)
There are more references available in the full text version of this article.

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