Free radical scavenging activities of mushroom polysaccharide extracts
Abstract
The superoxide and hydroxyl radical scavenging activities of eight mushroom antitumor polysaccharide extracts were investigated using phenazin methosulphate-NADH-nitroblue tetrazolium system and ascorbic acid-Cu2+-cytochrome C system respectively. The results showed that six of eight mushroom polysaccharide extracts had superoxide and hydroxyl radical scavenging activities. The protein content of the polysaccharide extracts appeared to contribute a direct effect on free radical scavenging activity. However, none of the mushroom polysaccharide extracts had antioxidative activity as measured by detecting malondialdehyde (MDA) contents of liver microsomes.
References (26)
- T. Miyazaki et al.
Carbohydr. Res.
(1978) - E. Kishida et al.
Carbohydr. Res.
(1989) - F. Liu et al.
Life Sci.
(1996) - K. Tabata et al.
Carbohydr. Res.
(1981) - O.M. Lowry et al.
J. Biol. Chem.
(1951) - P.F. Wang et al.
Biochem. Pharmacol.
(1996) - J.L. Marx
Science
(1987) - R.L. Zhang et al.
Scientia Sinica
(1988) - T.A. Sarafian et al.
Free Rad. Res.
(1994) - Y.C. Zhou et al.
Biochem. Pharmacol.
(1991)
Planta Med.
Acta Biochimica and Biophysica Sinica
Anticancer Res.
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