Delayed eosinophil programmed cell death in vitro: A common feature of inhalant allergy and extrinsic and intrinsic atopic dermatitis☆,☆☆,★,★★
Section snippets
Reagents
Human recombinant GM-CSF (6.85 × 108 U/mg), IL-3 (6.9 × 107 U/mg), and IL-5 (4 × 107 U/mg) were obtained from Genzyme (Cambridge, Mass.). Before testing, all stimulating reagents were diluted in control medium (Hanks balanced salt solution [HBSS] containing 1 mg/ml bovine serum albumin). Dynabeads M-450 sheep anti-mouse IgG were purchased from Dynal A.S. (Oslo, Norway). All monoclonal antibodies were purchased from Immunotech (Marseille, France) except for CD69, which was from Becton Dickinson
Eosinophil count and total IgE level
Patients with AD (mean eosinophil count ± SEM was 16.7 ± 1.7% in ADe type and 10.4 ± 2.5% in ADi) and IA (eosinophil count, 17.6 ± 2.7%) did not show increased absolute eosinophil numbers when compared to NA volunteers (eosinophil count, 14.2 ± 1.6%).
In contrast, total IgE levels were significantly higher in patients with ADe (geometric mean, 2989.5 kU/L) as compared to NA subjects (15.5 kU/L) (p < 0.0001), patients with IA (286.6 kU/L) (p < 0.05), and patients with ADi (22.2 kU/L) (p < 0.005).
Effect of culture on eosinophil viability
Discussion
The data presented clearly demonstrate by qualitative and quantitative assays that PCD is significantly delayed in eosinophils of subjects with IA and particularly in those with AD, as compared to NA controls. Furthermore, the results suggest that delayed PCD may be mediated at least in part by an autocrine production of GM-CSF and probably of IL-5.
The demonstrated survival rates of eosinophils from NA subjects in culture are consistent with published work showing low viability in healthy
References (55)
- et al.
Atopic dermatitis: an update
J Am Acad Dermatol
(1996) - et al.
Atopic dermatitis: is it an allergic disease?
J Am Acad Dermatol
(1995) - et al.
House dust mite-specific T cells in the skin of subjects with atopic dermatitis: frequency and lymphokine profile in the allergen patch test
J Allergy Clin Immunol
(1992) Eosinophils in atopic dermatitis
J Allergy Clin Immunol
(1994)- et al.
Biochemical properties of eosinophils and their preferential accumulation mechanism in nasal allergy
J Allergy Clin Immunol
(1994) - et al.
Skin eosinophilia in patients with allergic and nonallergic asthma and atopic dermatitis
J Allergy Clin Immunol
(1992) - et al.
Eosinophil cationic protein (ECP) in sera of patients with atopic dermatitis
J Am Acad Dermatol
(1991) - et al.
Secretion of granule proteins from eosinophils and neutrophils is increased in asthma
J Allergy Clin Immunol
(1991) - et al.
High dose UVA1 therapy in the treatment of patients with atopic dermatitis
J Am Acad Dermatol
(1992) - et al.
Peripheral blood eosinophilia in infants at 3 months of age is associated with subsequent development of atopic disease in early childhood
J Allergy Clin Immunol
(1995)
Analysis of the survival of mature human eosinophils: interleukin-5 prevents apoptosis in mature human eosinophils
Blood
The chemokine RANTES is more than a chemoattractant: characterization of its effect on human eosinophil oxidative metabolism and morphology in comparison with IL-5 and GM-CSF
J Invest Dermatol
Induction of apoptosis in human eosinophils by anti-Fas antibody treatment in vitro
Blood
Fas-mediated apoptosis in cultured human eosinophils
Blood
Eosinophils 1992
Immunol Today
Interleukin-5 messenger RNA and immunoreactive protein expression by activated eosinophils in lesional atopic dermatitis skin
J Invest Dermatol
Cytokine generation by eosinophils
J Allergy Clin Immunol
In vivo expression of CD69 on lung eosinophils in eosinophilic pneumonia: CD69 a possible activation marker for eosinophils
J Allergy Clin Immunol
Ligation of CD69 induces apoptosis and cell death in human eosinophils
Blood
Inhibition of programmed cell eosinophil death: a key pathogenic event for eosinophilia?
Immunol Today
Atopic dermatitis: the skin manifestation of atopy
Clin Exp Allergy
Human atopen-specific types 1 and 2 T helper cell clones
J Immunol
Eosinophilic inflammation in asthma
N Engl J Med
Current understanding of the role of the eosinophil granulocyte in asthma
Clin Exp Allergy
Eosinophils in allergy: role in disease, degranulation, and cytokines
Int Arch Allergy Immunol
Dermal deposition of eosinophil-granule major basic protein in atopic dermatitis
N Engl J Med
Cutaneous late-phase response to allergen: mediator release and inflammatory cell infiltration
J Clin Invest
Cited by (142)
Intrinsic atopic dermatitis shows similar T<inf>H</inf>2 and higher T <inf>H</inf>17 immune activation compared with extrinsic atopic dermatitis
2013, Journal of Allergy and Clinical ImmunologyEosinophil Cell-Cell Communication
2013, Eosinophils in Health and DiseaseEosinophil Cell–Cell Communication
2012, Eosinophils in Health and DiseaseSalbutamol delays human eosinophil apoptosis via a cAMP-dependent mechanism
2011, Pulmonary Pharmacology and TherapeuticsCitation Excerpt :In vitro and in vivo eosinophil apoptosis is delayed by cytokines such as interleukin-5 and granulocyte-macrophage colony-stimulating factor and enhanced by glucocorticoids and death receptors i.e. Fas [2–4]. We, and others, have previously shown that eosinophil apoptosis is delayed in patients with asthma or inhalant allergy [7–9]. Bronchoconstriction in asthma or COPD is commonly relieved with β2-agonists.
Potent Anti-Inflammatory Effects of Tetracyclines on Human Eosinophils
2021, Frontiers in AllergyApoptosis of eosinophil granulocytes
2020, Biology
- ☆
From the Department of Dermatology, Hannover Medical School, Hannover.
- ☆☆
Supported by research grant We-1912/1-1 and Ka-578/7-1 from the Deutsche Forschungsgemeinschaft.
- ★
Reprint requests: Bettina Wedi, MD, Department of Dermatology, Hannover Medical School, Ricklinger Str. 5, D-30449 Hannover, Germany.
- ★★
1/1/83666