Elsevier

Methods in Cell Biology

Volume 52, 1997, Pages 261-264, 264a, 264b, 264c, 264d, 264e, 264f, 264g, 264h, 264i, 264j, 265-272
Methods in Cell Biology

Chapter 12 Methods for Myoblast Transplantation

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Publisher Summary

This chapter focuses on the techniques of transplanting myoblasts derived from primary cultures of mouse muscle. Myoblast transplantation provides a unique means to effect in vivo gene transfer because of the ability of implanted myoblasts to fuse with host myofibers. Among the limitations to more widespread use of myoblast transplantation is the time required for growth, purification, and labeling of cells and for detailed transplant analysis. For most applications of myoblast transplantation, it is essential to have a method of differentiating the donor cells from the host cells, whether to determine the fate of the transplanted cells, to assess the effect on host fibers of the incorporation of donor cells, or simply to assess the efficiency of the transplantation procedure. Retroviral-mediated gene transfer offers a rapid and efficient method of introducing a hereditable marker into dividing cells.

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