Short communicationCloning and characterization of Leishmania tarentolae adenine phosphoribosyltransferase
Section snippets
The biosynthesis of purine, pyridine and pyrimidine nucleotides is catalyzed by enzymes of the phosphoribosyltransferase (PRTase) family. Most organisms synthesize adenine nucleotides by both the de novo and the salvage pathways. In contrast, all protozoan parasites examined to date are strict purine nucleotide auxotrophs because of the absence of a purine de novo biosynthetic pathway [1]. Kinetoplastid protozoa from the genus Leishmania possess three enzymes involved in the recycling of purine
Acknowledgements
The work was supported in part by a research grant AI-09102 to L.S. from the National Institutes of Health. We would like to thank the members of the Simpson laboratory for helpful discussions in the course of this work. We would like to express our gratitude to Dr James A. Lake for helpful discussion on the phylogeny of the APRT genes and to Dr Beatriz D. Lima for the L. tarentolae λZAP express genomic library.
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