In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells
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Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
2018, Developmental CellCitation Excerpt :We speculate that chemical and physical coupling of SM synthesis, DAG production, Ca2+ influx, and capture of secretory cargo by Cab45 promotes engulfment of oligomeric Cab45-client complexes by TGN membrane, leading to vesicle budding (Figure 7). As no integral membrane receptor(s) for Cab45 clients has been identified, we suggest the formation of secretory carriers of the SMS pathway carrying Cab45-client complexes resembles that of zymogen granule formation in pancreatic acinar cells, where the enzymes are proposed to form a “submembrane matrix” that deforms the membrane to induce budding of a secretory granule from the TGN (Dartsch et al., 1998; Schmidt et al., 2000). Thus, a common mechanism may be employed for the formation of secretory carriers containing different pools of proteins destined for secretion.
Molecular basis of diseases of the exocrine pancreas
2018, Molecular Pathology: The Molecular Basis of Human DiseaseModulating zymogen granule formation in pancreatic AR42J cells
2012, Experimental Cell ResearchCitation Excerpt :The variety of pancreatic digestive enzymes is packaged in a condensed and predominantly inactive form into large (approx. 1 μm in diameter) secretory organelles, so called zymogen granules (ZG), which are released by regulated apical secretion, triggered by external stimuli. ZG formation is initiated at the trans-Golgi network (TGN) where the regulated secretory ZG proteins co-aggregate at the mildly acidic pH and high calcium levels and condensing vacuoles/immature secretory granules are formed [1–4]. They mature by further concentration of the cargo proteins with selective removal of components not destined for regulated secretion.
Serglycin proteoglycan is not implicated in localizing exocrine pancreas enzymes to zymogen granules
2009, European Journal of Cell BiologyMolecular Basis of Diseases of the Exocrine Pancreas
2009, Molecular Pathology: The Molecular Basis of Human DiseaseAbsence of the major zymogen granule membrane protein, GP2, does not affect pancreatic morphology or secretion
2004, Journal of Biological Chemistry
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Dr. Horst E Kern, Institut für Zytobiologie and Zytopathologie, Robert-Koch-Str. 5, D-35033 Marburg/Lahn.