Elsevier

Neuroscience Letters

Volume 242, Issue 1, 6 February 1998, Pages 25-28
Neuroscience Letters

Characterization of the 5′ region of the rat brain glycine transporter GLYT2 gene: identification of a novel isoform

https://doi.org/10.1016/S0304-3940(98)00037-8Get rights and content

Abstract

We have identified two alternative 5′ ends for the GLYT2 glycine transporter in rat brain DNA by using rapid amplification of cDNA ends (RACE) analysis. Study of the genomic DNA revealed that the isoform diversity is generated by alternative use of exons Ia or Ib, respectively. Upon translation, the mRNA corresponding to the novel isoform GLYT2b would yield a protein five amino acids longer than the previously characterized isoform GLYT2a. Both forms display similar regional distribution and kinetics characteristics. However, whereas GLYT2a is able to actively accumulate glycine into transfected COS cells, GLYT2b seems only to exchange (or release) glycine.

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Acknowledgements

The authors thank E. Nuñez for excellent technical assistance. This work was supported by grants from the Spanish Dirección General de Investigación Cientı́fica y Técnica (PM95-0026), Boehringer Ingelheim España S.A., the BIOMED program of the European Union (BMH1-CT93-1110 and BMH4-CT95-0571) and an institutional grant from the Fundación Ramón Areces.

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