Regulation of FAT/CD36 gene expression: further evidence in support of a role of the protein in fatty acid binding/transport

doi:10.1016/S0952-3278(97)90487-7

Prostaglandins, Leukotrienes and Essential Fatty Acids

Volume 57, Issue 1, July 1997, Pages 17-21

https://doi.org/10.1016/S0952-3278(97)90487-7 Get rights and content

Abstract

Much biochemical evidence has implicated rat adipocyte CD36 (FAT) in membrane binding and transport of long-chain fatty acids (FA). Expression of the mRNA favored tissues with active FA metabolism and was upregulated in vivo with diabetes and with high fat feeding. In culture, CD36 mRNA was a strong marker of preadipocyte differentiation and was modulated by the same factors effective on mRNAs coding for other proteins involved in FA metabolism. In preadipocytes, long-chain FA or 2-bromopalmitate but not short-chain FA strongly induced CD36 mRNA within 8 h to an optimum within 24 h. Removal of the FA resulted in a decay of CD36 mRNA with a half life of about 12 h. In differentiated adipocytes, levels of CD36 mRNA were downregulated by the 3′:5′-cyclic adenosine monophosphate, cAMP, analog, 8-(4-chlorophenylthio) adenosine, 8-CPT, at concentrations of 1–100 μM. The effect, observed within 6 h, was optimal after 18 h and independent of the action of 8-CPT to mobilize FA. Regulation of CD36 expression by factors effective on expression of other proteins implicated in FA metabolism is consistent with its role in membrane FA transport.

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Original articleRegulation of FAT/CD36 gene expression: further evidence in support of a role of the protein in fatty acid binding/transport

Abstract

J Biol Chem

J Biol Chem

Cell

Blood

Biochem Biophys Res Commun

J Biol Chem

Hepatocellular uptake of oleate is energy dependent, sodium linked and inhibited by an antibody to a hepatocyte membrane protein

Purification of high affinity acid receptors in rat myocardial sarcolemmal membranes

Lipids

Photo affinity labeling and fatty acid permeation in 3T3-L1 adipocytes

J Biol Chem

Binding of sulfosuccinimidyl fatty acids to adipocyte membrane proteins: isolation and amino-terminal sequence of an 88 kDa protein implicated in transport of fatty acids

J Membrane Biol

Expression cloning and characterization of a novel adipocyte long chain FA transport protein

Cell

Original article
Regulation of FAT/CD36 gene expression: further evidence in support of a role of the protein in fatty acid binding/transport