Combined in situ hybridization and immunocytochemistry in the assay of pharmacological effects on tyrosine hydroxylase mRNA concentration
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2000, Brain ResearchCitation Excerpt :Control brain slides were hybridized, exposed and developed simultaneously with the experimental slides. Uniformly these control slides demonstrated the known patterns of gene expression in striatopallidal neurons for A2a-adenosine and D2-dopamine receptor [39,15], cortical neurons for A1-adenosine receptors [40], and nigrostriatal neurons for TH [31] (data not shown). In addition, the patterns of TH and D2-dopamine, A2a-adenosine and A1-adenosine receptor mRNA expression differed in the tissue bloc of the carotid body and petrosal ganglion, further demonstrating probe-specificity.
Lack of induction of substance P gene expression by hypoxia and absence of neurokinin 1-receptor mRNAs in the rat carotid body
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