Expression pattern of the Rsk2, Rsk4 and Pdk1 genes during murine embryogenesis
Section snippets
Results and discussion
The 90 kDa ribosomal S6 family of serine/threonine kinases mediates cellular signaling downstream of the mitogen-activated protein kinase (MAPK) cascade. They are activated by extracellular regulated kinases (ERK1 and 2), Jun kinase, and other cellular stress stimuli. Consequently, a wide variety of cellular functions for RSK proteins has been proposed (Frödin and Gammeltoft, 1999). These range from cell cycle control to gene regulation by direct histone H3 phosphorylation and activation of
Experimental procedures
Embryos were isolated from pregnant C57Bl/6 mice at the times indicated and fixed in 4% paraformaldehyde in phosphate-buffered saline at 4 °C prior to cryostat sectioning. Ten-micrometer sections were mounted on slides. RNA in situ hybridization was performed on whole-body sections from embryos at 9.5–17.5 dpc and specific sections from 2-day-old mice. To investigate the expression pattern of the mouse Pdk1 gene, IMAGE clone 2648327 (AW323652) was used. The 1.9 kb insert of this clone spans the
Acknowledgements
This research project was supported by the Deutsche Forschungsgemeinschaft (HA1082/16-1, 16-2).
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