Reciprocal expression of peroxisome proliferator-activated receptor-γ and cyclooxygenase-2 in human term parturition☆
Section snippets
Subject recruitment
All procedures were conducted under a protocol approved by the Institutional Review Board of The Ohio State University Medical Center, and informed consent was obtained from all women. Placentas with attached fetal membranes were collected from women with uncomplicated singleton pregnancies following term (≥37 weeks of gestation), spontaneous labor (SL), or cesarean section (CS) without labor. The tissues were transported to our laboratory within 30 minutes following delivery in 0.9% NaCl.
COX-2 and PPAR-γ are reciprocally expressed in term fetal membranes after labor
The mean gestational age at delivery for the TL group was 39.6 weeks (range, 38.4-41.1 weeks), which was similar to that for the CS group (38.9 weeks; range, 37.1-40.3 weeks).
Other clinical and demographic data did not differ appreciably between the groups (Table). Women in the TL group were recruited after presenting with regular uterine contractions accompanied by changes in cervical dilation or effacement. Women undergoing induction of labor were excluded, although women undergoing
Comment
The observation of increased levels of inflammatory cytokines (interleukin-1, interleukin-6, interleukin-8, and tumor necrosis factor) within intrauterine tissues after labor indicates that inflammation plays a significant role in term human parturition.22 As autocrine and paracrine mediators, these cytokines in turn stimulate uterotonic PG formation, which is achieved primarily via de novo COX-2 expression.1., 2., 3. PGs, in turn, facilitate cervical maturation and myometrial contractility.3
Acknowledgements
We thank Valerie J. Curry, RN, for assistance in patient enrollment and specimen collection. We additionally thank Peter Zimmerman (The Ohio State University, Department of Pathology) and Susan Jones (The Ohio State University Histology Core Facility) for excellent technical support. This work was performed while Dr Lisa Dunn-Albanese was a Fellow in maternal-fetal medicine at The Ohio State University. This work was supported by National Institutes of Health HD35881 and The Ohio State
References (29)
- et al.
Pathogenesis of preterm labor and preterm premature rupture of membranes associated with intraamniotic infection
Infect Dis Clin North Am
(1997) - et al.
Regulation of parturition update. Endocrine and paracrine effectors of term and preterm labor
Clin Perinatol
(1998) - et al.
An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma)
J Biol Chem
(1995) - et al.
PPARgamma : a nuclear regulator of metabolism, differentiation, and cell growth
J Biol Chem
(2001) - et al.
15-Deoxy-delta 12, 14-prostaglandin J2 is a ligand for the adipocyte determination factor PPAR gamma
Cell
(1995) - et al.
The 15-deoxy-delta(12,14)-prostaglandin J(2)receptor, peroxisome proliferator activated receptor-gamma (PPARgamma) is expressed in human gestational tissues and is functionally active in JEG3 choriocarcinoma cells
Placenta
(2000) - et al.
Expression of PPAR and RXR isoforms in the developing rat and human term placentas
Placenta
(2002) - et al.
PPAR gamma is required for placental, cardiac, and adipose tissue development
Mol Cell
(1999) - et al.
Peroxisome proliferator-activated receptor gamma ligands suppress the transcriptional activation of cyclooxygenase-2. Evidence for involvement of activator protein-1 and CREB-binding protein/p300
J Biol Chem
(2001) - et al.
15-lipoxygenase-1 metabolites down-regulate peroxisome proliferator-activated receptor gamma via the MAPK signaling pathway
J Biol Chem
(2001)
Cytokine abundance in placental tissues: evidence of inflammatory activation in gestational membranes with term and preterm parturition
Am J Obstet Gynecol
Endogenous PPAR gamma mediates anti-inflammatory activity in murine ischemia-reperfusion injury
Gastroenterology
Effective diminution of amniotic prostaglandin production by selective inhibitors of cyclooxygenase type 2
Am J Obstet Gynecol
Control of human parturition
Semin Perinatol
Cited by (43)
Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition
2022, Journal of Lipid ResearchExpression of 15-Hydroxyprostaglandin Dehydrogenase in Human Chorion Is Associated with Peroxisome Proliferator-Activated Receptor Isoform Expression in Term Labor
2015, American Journal of PathologyCitation Excerpt :Berry et al21 have demonstrated the data from relative larger samples (n = 15 in each group) that PPARα mRNA in choriodecidua decreases after onset of labor. Dunn-Albanese et al37 reported that the protein amount of PPARγ significantly decreases in fetal membranes with labor at term, but they did not distinguish the amount of PPARγ in amnion and choriodecidua. Here, we recruited more patients (n = 24 in each group) and found that PPARγ and PPARβ protein expression significantly decreased in chorion laeve with labor at term.
Peroxisome proliferator-activated receptor-gamma in normal human pregnancy and miscarriage
2009, Acta HistochemicaPeroxisome Proliferator-activated Receptors and Retinoid X Receptor-alpha in Term Human Gestational Tissues: Tissue Specific and Labour-associated Changes
2009, PlacentaCitation Excerpt :Currently available data indicates that PPARs may either have roles in the mechanisms that maintain pregnancy or initiate human labour at term [3,9,13–15]. These studies have been limited in their interpretation of the exact role of PPARs and RXRα in human parturition because samples from Caesarean section deliveries (not-in-labour) were compared to samples obtained after term labour (from term spontaneous vaginal deliveries) [13,14,16,17]. To date, there are no data available comparing these groups to samples obtained from term women in active labour.
Effect of placental fatty acid metabolism and regulation by peroxisome proliferator activated receptor on pregnancy and fetal outcomes
2007, Journal of Pharmaceutical SciencesImpact of PUFA on early immune and fetal development
2008, British Journal of Nutrition
- ☆
This work was supported by National Institutes of Health HD35881 and The Ohio State University Perinatal Research and Development Fund.
Dr Dunn-Albanese and Dr Ackerman contributed equally to this work.