Biochemical and Biophysical Research Communications
Src tyrosine kinase promotes survival and resistance to chemotherapeutics in a mouse ovarian cancer cell line
Section snippets
Methods
Reagents. All liquid media were purchased from Life Technologies (Grand Island, NY). A Src specific antibody (clone 327) was purchased from Oncogene Science (Cambridge, MA). A phospho-Src specific antibody that recognizes tyrosine 418 phosphorylated Src was purchased from Biosource International (Camarillo, CA). A Ras activation assay kit and FAK antibodies were from Upstate Biotechnology (Lake Placid, NY). Akt, phospho-Akt, and phospho-FKHR were from Cell Signaling (Beverly, MA). A
ID8 cells express constitutively active Src tyrosine kinase
Because Src tyrosine kinase has been reported to be overexpressed and activated in both human ovarian cancer cell lines and late stage ovarian cancers [8], Src protein and kinase activity were determined in ID8 mouse ovarian cancer cells and ID8 cell derived tumors. ID8 cells express a significant amount of Src protein in comparison to early passage human ovarian surface epithelial cells (Fig. 1A). ID8 mouse ovarian cancer cells from serum-starved cultures express comparable levels of Src
Acknowledgements
This research was supported by National Institutes of Health Grants HD36013 (Chris Taylor), CA50616 (Paul Terranova) and Department of Defense Grant OC990038 (Chris Taylor).
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