Biochemical and Biophysical Research Communications
Marked change in microRNA expression during neuronal differentiation of human teratocarcinoma NTera2D1 and mouse embryonal carcinoma P19 cells
Section snippets
Materials and methods
Cell culture and induction of differentiation. NTera2D1 and Neuro2a (N2a) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Wako) supplemented with 10% fetal bovine serum (Sigma), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma), as previously described [20], [21]. PC12D cells were grown in DMEM supplemented with 10% fetal bovine serum, 5% horse serum and the antibiotics mentioned above. P19 cells were grown in α-MEM (Wako) supplemented with 10% fetal bovine serum and the
Marked change in miRNA expression during neuronal differentiation of NTera2D1 and P19 cells
NTera2D1, P19, Neuro2a (N2a) and PC12D cells can be induced into differentiated cells with long neuritic processes (Fig. 1) which look very similar to neuronal cells. To see the relationship between the differentiation and miRNAs, we carried out expression profile analysis of miRNAs during neuronal differentiation of the cells by means of the Genopal-MICH and -MICM DNA chips (Mitsubishi Rayon). The Genopal is a new type of DNA chip composed of plastic hollow fibers, and oligonucleotide DNA
Acknowledgments
We thank Y. Nishiura and H. Oshima for their assistance. We also thank Dr. Y. Nagai for providing PC12D cells and for his helpful advice on cell culture. This work was supported in part by research grants from the Ministry of Health, Labour and Welfare of Japan and by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.
References (24)
MicroRNAs: genomics, biogenesis, mechanism, and function
Cell
(2004)- et al.
RAS is regulated by the let-7 microRNA family
Cell
(2005) - et al.
Identification of tissue-specific microRNAs from mouse
Curr. Biol.
(2002) - et al.
Embryonic stem cell-specific MicroRNAs
Dev. Cell
(2003) - et al.
Expression profile analysis of microRNA (miRNA) in mouse central nervous system using a new miRNA detection system that examines hybridization signals at every step of washing
Gene
(2007) - et al.
Gene silencing analyses against amyloid precursor protein (APP) gene family by RNA interference
Cell Biol. Int.
(2006) - et al.
Human microRNA clusters: genomic organization and expression profile in leukemia cell lines
Biochem. Biophys. Res. Commun.
(2006) - et al.
Human embryonic stem cells express a unique set of microRNAs
Dev. Biol.
(2004) - et al.
The nuclear RNase III Drosha initiates microRNA processing
Nature
(2003) - et al.
Processing of primary microRNAs by the Microprocessor complex
Nature
(2004)
A microRNA in a multiple-turnover RNAi enzyme complex
Science
A microRNA array reveals extensive regulation of microRNAs during brain development
RNA
Cited by (57)
Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain
2018, Molecular Therapy Nucleic AcidsCitation Excerpt :Thousands of miRNA genes have been found in animals and plants (see the microRNA database [miRBase]: http://www.mirbase.org/index.shtml). Expression profiles of miRNAs have been examined, and tissue- and stage-specific expression of miRNAs and disease-associated expression of miRNAs have been detected.4–10 Thus, miRNAs may become useful biomarkers and may provide us with clues to help better disease treatment.9–11
Effects of pore orientation on in-vitro properties of retinoic acid-loaded PLGA/gelatin scaffolds for artificial peripheral nerve application
2017, Materials Science and Engineering C