Journal of Molecular Biology
Volume 387, Issue 5, 17 April 2009, Pages 1061-1066
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Communication
Carbohydrate and Domain Architecture of an Immature Antibody Glycoform Exhibiting Enhanced Effector Functions

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Abstract

Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.

Section snippets

Protein Data Bank accession number

Coordinates and structure factors of the Man9GlcNAc2 glycoform of human IgG1-Fc have been deposited in the Protein Data Bank with accession number 2WAH.

Acknowledgements

We thank W. Lu for technical assistance with tissue culture and the staff of BM-14 at the European Radiation Synchrotron Facility. This work was funded by Cancer Research UK, the UK Medical Research Council, the European Commission as SPINE2 Complexes (FP6-RTD-031220), and the Wellcome Trust. We also thank the Wellcome Trust and the Biotechnology and Biological Sciences Research Council for equipment grants to the Glycobiology Institute to purchase the Q-Tof and TofSpec mass spectrometers,

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    M.C. and T.A.B. contributed equally to this work.

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