Elsevier

Developmental Biology

Volume 302, Issue 2, 15 February 2007, Pages 399-411
Developmental Biology

CYP26A1 and CYP26C1 cooperatively regulate anterior–posterior patterning of the developing brain and the production of migratory cranial neural crest cells in the mouse

https://doi.org/10.1016/j.ydbio.2006.09.045Get rights and content
Under an Elsevier user license
open archive

Abstract

The appropriate regulation of retinoic acid signaling is indispensable for patterning of the vertebrate central nervous system along the anteroposterior (A–P) axis. Although both CYP26A1 and CYP26C1, retinoic acid-degrading enzymes that are expressed at the anterior end of the gastrulating mouse embryo, have been thought to play an important role in central nervous system patterning, the detailed mechanism of their contribution has remained largely unknown. We have now analyzed CYP26A1 and CYP26C1 function by generating knockout mice. Loss of CYP26C1 did not appear to affect embryonic development, suggesting that CYP26A1 and CYP26C1 are functionally redundant. In contrast, mice lacking both CYP26A1 and CYP26C1 were found to manifest a pronounced anterior truncation of the brain associated with A–P patterning defects that reflect expansion of posterior identity at the expense of anterior identity. Furthermore, Cyp26a1−/−Cyp26c1−/− mice fail to produce migratory cranial neural crest cells in the forebrain and midbrain. These observations, together with a reevaluation of Cyp26a1 mutant mice, suggest that the activity of CYP26A1 and CYP26C1 is required for correct A–P patterning and production of migratory cranial neural crest cells in the developing mammalian brain.

Keywords

Central nervous system
CYP26
Anteroposterior axis
Neural crest cells
Retinoic acid

Cited by (0)

1

Current address: Internal Medicine and Cell and Developmental Biology, 3131 CCGC, University of Michigan, 1440E Medical Center Drive, 210 Washtnaw Avenue 5382 LSI, Ann Arbor, MI 48109, USA.

2

Current address: Department of Plastic Surgery, Fujita Health University, School of Medicine, 1-98 Dengakugakubo, Kutukake-cho, Toyoake, Aichi 470-1192, Japan.