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The mouse ERG before and after light damage is independent of p53

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Abstract

Death of retinal photoreceptors by apoptosis is observed under many physiological and pathological conditions such as histogenesis, retinal dystrophies and light-induced photoreceptor degeneration. To date, little is known about regulatory mechanisms for apoptosis in the retina. The tumor suppressor gene p53 is a regulator of apoptosis in a number of systems, however, p53-independent apoptosis has also been described. We have therefore investigated whether the lack of p53 influences the dark-adapted ERG in C57BL/6 p53−/− mice compared to p53+/+ control littermates under physiological (regular light-dark cycle) conditions. We also recorded ERGs at 12 to 14 h in darkness following diffuse bright light exposure to 8′000 or 15'000 lux for 2 h. ERG analysis over a range of 6 logarithmic units of light intensity revealed normal and virtually identical a-, b-, c-waves and oscillatory potentials in dark-adapted p53+/+ and p53−/− mice. After exposure to diffuse white fluorescent light strong decreases of all ERG components were found to be very similar in both genotypes. These data support the notion that the p53 protein is neither essential for normal retinal function nor for processes involved in light-induced depression of the ERG in mice.

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References

  1. Heckenlively JR, Winston JV, Roderick TH. Screening for mouse retinal degenerations. I. Correlation of indirect ophthalmoscopy, electroretinograms, and histology. Doc Ophthalmol 1989; 71(3): 229-39.

    Article  PubMed  CAS  Google Scholar 

  2. Peachey NS, Goto Y, Quiambao AB, al Ubaidi MR. Functional consequences of oncogene-induced photoreceptor degeneration in transgenic mice. Vis Neurosci 1995; 12(3): 513-22.

    Article  PubMed  CAS  Google Scholar 

  3. Smith SB, Hainasaki DI. Electroretinographic study of the C57BL/6-mivit/mivit mouse model of retinal degeneration. Invest Ophthalmol Vis Sci 1994; 35(7): 3119-23.

    PubMed  CAS  Google Scholar 

  4. Jacobson SG, Cideciyan AV, Kemp CM, Sheffield VC, Stone EM. Photoreceptor function in heterozygotes with insertion or deletion mutations in the RDS gene. Invest Ophthalmol Vis Sci 1996; 37(8): 1662-74.

    PubMed  CAS  Google Scholar 

  5. Gorman AM, McGowan A, Oneill C, Cotter T. Oxidative Stress and Apoptosis In Neurodegeneration. J Neurol Sci 1996; 139(Suppl S): 45-52.

    Article  PubMed  Google Scholar 

  6. Thompson GB. Apoptosis in the pathogenesis and treatment of disease. Science 1995; 267(5203): 1456-62.

    PubMed  CAS  Google Scholar 

  7. Young RW. Cell death during differentiation of the retina in the mouse. J Comp Neurol 1984; 229: 362-373.

    Article  PubMed  CAS  Google Scholar 

  8. Chang GQ, Hao Y, Wong F. Apoptosis: final common pathway of photoreceptor death in rd, rds, and rhodopsin mutant mice. Neuron 1993; 11(4): 595-605.

    Article  PubMed  CAS  Google Scholar 

  9. Portera-Cailliau C, Sung CH, Nathans J, Adler R. Apoptotic photoreceptor cell death in mouse models of retinitis pigmentosa. Proc Natl Acad Sci 1994; 91(3): 974-8.

    Article  PubMed  CAS  Google Scholar 

  10. Li Z-Y, Milam A. Apoptosis in retinitis pigmentosa. in: Anderson RE, LaVail MM, Hollyfield JG, Editors. Degenerative Diseases of the Retina. New York and London.: Plenum Press, 1995: 1-8.

    Google Scholar 

  11. Hafezi F, Steinbach JP, Marti A, Munz K, Wang ZQ, Wagner EF, Aguzzi A, Remé ChE. The absence of c-fos prevents light-induced apoptotic cell death of photoreceptors in retinal degeneration in vivo. Nat Med 1997; 3(3): 346-9.

    Article  PubMed  CAS  Google Scholar 

  12. Hafezi F, Marti A, Munz K, Remé ChE. Light-induced apoptosis: differential timing in the retina and pigment epithelium. Exp Eye Res. 1997; 64: 963-70.

    Article  PubMed  CAS  Google Scholar 

  13. Donehower LA, Bradley A. The tumor suppressor p53. Biochem Biophys Acta 1993; 1155(2): 181-205.

    PubMed  CAS  Google Scholar 

  14. Donehower LA, Harvey M, Slagle BL, McArthur MJ, Montgomery CA, Jr., Butel JS, Bradley A. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumors. Nature 1992; 356(6366): 215-21.

    Article  PubMed  CAS  Google Scholar 

  15. Clarke AR, Purdie CA, Harrison Di, Morris RG, Bird CC, Hooper ML, Wyllie AH. Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature 1993; 362(6423): 849-52.

    Article  PubMed  CAS  Google Scholar 

  16. Papermaster DS. Apoptosis Of the Mammalian Retina and Lens. Cell Death Diff 1997; 4 (1): 21-28.

    Article  CAS  Google Scholar 

  17. Marti A, Hafezi F, Lansel N, Hegi M, Wenzel A, Grimm C, Niemeyer G, Remé ChE. Light-Induced Cell Death of Retinal Photoreceptors in the Absence of p53. Invest Ophthalmol Vis Sci 1998; 39(5): 846-9.

    PubMed  CAS  Google Scholar 

  18. Lansel N, Marti A, Hafezi F, Remé ChE, Niemeyer G. Electroretinography in wildtype and P53-/-knockout mice before and after light exposure. Invest Ophthalmol Vis Sci 1997; 38: S321.

    Google Scholar 

  19. Niemeyer G, Lansel N, Küng N, Marti A, Hafezi F, Remé ChE. Effects of deletion of the p53 gene on the ERG before and after light exposure. (35th symposium of ISCEV, Monterey, California, July 20-24, 1997).

  20. Niemeyer G, Küng N. A simple and stable DC-electrode for ocular electrophysiology. Documenta Ophthalmol 1998; 95: 55-61.

    Article  CAS  Google Scholar 

  21. Kaelin-Lang A, Niemeyer G. A PC program for evaluating electrophysiologic signals of mammalian eyes. Klin Monatsbl Augenheilk 1995; 206(5): 394-6.

    Article  CAS  Google Scholar 

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Lansel, N., Hafezi, F., Marti, A. et al. The mouse ERG before and after light damage is independent of p53. Doc Ophthalmol 96, 311–320 (1998). https://doi.org/10.1023/A:1001795526628

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  • DOI: https://doi.org/10.1023/A:1001795526628

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