Abstract
IN vertebrates, fully differentiated cells such as skeletal muscle and some nerve cells are highly sensitive to acetyl-choline (ACh), due to the presence of acetylcholine receptors in their surface membranes. In muscle fibres, the ACh-sensitivity is known to appear early during differentiation1–3, but relatively little is known about the chemical sensitivity of the membrane in the undifferentiated cell. In particular, it would be interesting to know if spermatozoa and oocytes are already sensitive to ACh and other neurotransmitter substances. To examine this question, toad (Xenopus laevis) oocytes were taken from the ovary, kept in frog's Ringer or Merriam4 solution, and studied at room temperature, using conventional electrophysiological techniques.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Diamond, J. & Miledi, R. J. Physiol., Lond. 162, 393–408 (1962).
Ritchie, A. K. & Fambrough, D. M. J. gen. Physiol. 65, 327–356 (1975).
Blackshaw, S. & Warner, A. Nature 262, 217–218 (1976).
Merriam, R. W. Expl Cell Res. 68, 81 (1971).
Moreau, M., Guerrier, P. & Dorée, M. C. r. hebd. Séanc. Acad. Sci. Paris D 282, 1309–1312 (1976).
Ginsborg, B. L. Biochim. Biophys. Acta 300, 289–317 (1973).
Katz, B. & Miledi, R. Proc. R. Soc. Lond. B 161, 483–495 (1965).
Reshetnikova, N. A. Zh. Evol. Biochim. I. Fiziol. 6, 19–24 (1970).
Buznikov, G. A. Ontogenez 2, 5–13 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KUSANO, K., MILEDI, R. & STINNAKRE, J. Acetylcholine receptors in the oocyte membrane. Nature 270, 739–741 (1977). https://doi.org/10.1038/270739a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/270739a0
This article is cited by
-
Deep transcriptome profiling reveals limited conservation of A-to-I RNA editing in Xenopus
BMC Biology (2023)
-
On the excitation of action potentials by protons and its potential implications for cholinergic transmission
Protoplasma (2016)
-
Role of loop D of the α7 nicotinic acetylcholine receptor in its interaction with the insecticide imidacloprid and related neonicotinoids
British Journal of Pharmacology (2000)
-
Single-channel properties and regulation of pinacidil/glibenclamide-sensitive K+ channels in follicular cells from Xenopus oocyte
Pfl�gers Archiv European Journal of Physiology (1993)
-
Inositol trisphosphate may access calcium from stores not coupled to muscarinic receptors in Xenopus oocytes
Pfl�gers Archiv European Journal of Physiology (1992)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.