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Cloning of a human gene encoding the general transcription initiation factor IIB

Nature volume 352, pages 689–695 (1991)Cite this article

Abstract

Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic σ-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.

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Author notes

  1. William S. Lane: Harvard Microchemistry Facility, Harvard University, Cambridge, Massachusetts, USA

Authors and Affiliations

  1. Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, New Jersey, 08854-5635, USA

    Ilho Ha, William S. Lane & Danny Reinberg

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  1. Ilho Ha
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  2. William S. Lane
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  3. Danny Reinberg
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Ha, I., Lane, W. & Reinberg, D. Cloning of a human gene encoding the general transcription initiation factor IIB. Nature 352, 689–695 (1991). https://doi.org/10.1038/352689a0

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