Abstract
NITRIC oxide is an important bioregulatory molecule, being responsible, for example, for activity of endothelium-derived relaxing factor (EDRF)1–4. Acute hypertension5, diabetes6, ischaemia7and atherosclerosis8 are associated with abnormalities of EDRF. Nitric oxide is thought to be a retrograde messenger in the central nervous system9. The technology is not yet available for rapid detection of NO released by a single cell in the presence of oxygen and/or nitrite, so the release, distribution and reactivity of endogenous NO in biological systems cannot be analysed. Here we describe a porphyrinic microsensor that we have developed and applied to monitoring NO release in a microsystem. We selectively measured in situ the NO released from a single cell with a response time of less than 10 ms. The microsensor consists of p-type semiconducting polymeric porphyrin and a cationic exchanger (Nation) deposited on a thermally sharpened carbon fibre with a tip diameter of ∼0.5 (μm. The microsensor, which can be operated in either the amperometric or voltammetric mode, is characterized by a linear response up to 300 μM and a detection limit of 10 nM. Nitric oxide at the level of 10−20 mols can be detected in a single cell.
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References
Furchgott, R. F. & Zawadzki, J. V. Nature 288, 373–376 (1980).
Palmer, R. M. J., Ferrige, A. G. & Moncada, S. Nature 327, 524–526 (1987).
Furchgott, R. F. in Mechanism of Vasodilation Vol. 4 (ed. Vanhoutte, P. M.) 401–414 (Raven, New York, 1988).
Ignaro, L. J., Buga, G. M., Wood, K. S., Byrns, R. E. & Chaudhuri, G. Proc. natn. Acad. Sci. U.S.A. 84, 9265–9269 (1987).
Wei, E. P., Kontos, H. A., Christman, C. W., DeWitt, D. S. & Povlishock, J. T. Circ. Res. 57, 781–787 (1985).
Piper, G. M. & Gross, G. J. Am. J. Physiol. 24, 4825–4833 (1988).
Vanbethuysen, K. M., McMutry, I. F. & Horowitz, L. D. J. clin. Invest. 79, 265–274 (1987).
Freiman, P. C., Mitchall, G. C., Helstad, D. D., Armstrong, M. L. & Harrison, D. G. Circ. Res. 58, 783–789 (1986).
Schuman, E. M. & Madison, D. V. Science 254, 1503 (1991).
Bennett, J. E. & Mallnski, T. Chem. Mat. 3, 490–495 (1991).
Malinski, T., Ciszewski, A., Bennett, J., Fish, J. R. & Czuchajowski, L. J. Electrochem. Soc. 138, 2008–2015 (1991).
Kanner, J., Harel, S. & Granit, R. Arch. Biochem. Biophys. 289, 130–136 (1991).
Marletta, M. A. Trends Biochem. Sci. 14, 488–492 (1989).
Moncada, S., Palmer, M. J. & Higgs, E. A. Pharmac. Rev. 43, 109–142 (1991).
Rosenthal, A. M. & Gotlieb, A. T. in Cell Culture Techniques in Heart and Vessel Research (ed. Piper, H. M.) 117–139 (Springer, New York, 1990).
Downes, M. J., Edwards, M. W., Elsey, T. S. & Walters, C. L. Analyst 101, 742–748 (1978).
Shibuki, K. Neurosci. Res. 9, 69–76 (1990).
Gerhardt, G. A., Oke, A. F., Nagy, G., Moghaddam, B. & Adams, R. N. Brain Res. 290, 390–395 (1984).
Engstrom, R. C., Wightman, R. M. & Kristensen, E. W. Analyt. Chem. 60, 652–656 (1988).
Bailey, F., Malinski, T. & Kiechle, F. Analyt. Chem. 63, 395–398 (1991).
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Malinski, T., Taha, Z. Nitric oxide release from a single cell measured in situ by a porphyrinic-based microsensor. Nature 358, 676–678 (1992). https://doi.org/10.1038/358676a0
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DOI: https://doi.org/10.1038/358676a0
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