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Crystal structure of the p27Kip1 cyclin-dependent-kinase inibitor bound to the cyclin A–Cdk2 complex

Nature volume 382, pages 325–331 (1996)Cite this article

Abstract

The crystal structure of the human p27Kip1 kinase inhibitory domain bound to the phosphorylated cyclin A–cyclin-dependent kinase 2 (Cdk2) complex has been determined at 2.3 Å. p27Kip1 binds the complex as an extended structure interacting with both cyclin A and Cdk2. On cyclin A, it binds in a groove formed by conserved cyclin box residues. On Cdk2, it binds and rearranges the amino-terminal lobe and also inserts into the catalytic cleft, mimicking ATP.

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Authors and Affiliations

  1. Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York, 10021, USA

    Alicia A. Russo, Philip D. Jeffrey, Andrea K. Patten & Nikola P. Pavletich

  2. Cell Biology Program and the Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New York, New York, 10021, USA

    Joan Massagué

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  1. Alicia A. Russo
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  2. Philip D. Jeffrey
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  3. Andrea K. Patten
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  4. Joan Massagué
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  5. Nikola P. Pavletich
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Russo, A., Jeffrey, P., Patten, A. et al. Crystal structure of the p27Kip1 cyclin-dependent-kinase inibitor bound to the cyclin A–Cdk2 complex . Nature 382, 325–331 (1996). https://doi.org/10.1038/382325a0

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