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Cyclic nucleotide analogs as probes of signaling pathways

Nature Methods volume 5, pages 277–278 (2008)Cite this article

To the editor:

Cyclic AMP (cAMP) and cyclic GMP (cGMP) are critical second messengers that regulate multiple targets including different cAMP- or cGMP-dependent protein kinases (PKAs, PKGs)1,2, exchange proteins directly activated by cAMP (Epacs)3, phosphodiesterases (PDEs)4 and cyclic nucleotide-gated ion channels. Cyclic nucleotide analogs are widely used to study specificity of cellular signaling mediated by these target proteins. However, the selectivities and stabilities of these analogs need to be fully understood to properly interpret results and rigorously assess the mechanisms by which these analogs work in the cell.

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Acknowledgements

We thank J. Das and A. Intemann for technical assistance, M. Houslay (University of Glasgow) for mouse PDE4D3 cDNA, and J. Geiger for helping with the website. H.P. and E.B. were supported by Unibund Wuerzburg (Az. 05-43), J.A.B. and S.D.R. were supported by U.S. National Institutes of Health DK21723, Foundation Leducq, DFG-FOR 923 LU 1490/1-1 and ALA RG-52219N, H.R. and J.L.B. were funded by the Chemical Sciences of the Netherlands Organization for Scientific Research (NWO-CW).

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Author notes

  1. Heiko Poppe and Sergei D Rybalkin: These authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Clinical Biochemistry and Pathobiochemistry, University of Wuerzburg, Grombuehlstr. 12, Wuerzburg, 97080, Germany

    Heiko Poppe & Elke Butt

  2. Department of Pharmacology, University of Washington, 1959 NE Pacific St., Seattle, 98195, Washington, USA

    Heiko Poppe, Sergei D Rybalkin, Thomas R Hinds, Xiao-Bo Tang & Joseph A Beavo

  3. Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, Utrecht, 3584, CG, The Netherlands

    Holger Rehmann & Johannes L Bos

  4. Department of Ophthalmology, Haukeland University Hospital, Jonas Liesvei 65, Bergen, N-5021, Bergen, Norway

    Anne E Christensen

  5. Biolog Life Science Institute, Flughafendamm 9a, Bremen, 28199, Germany

    Frank Schwede & Hans-Gottfried Genieser

  6. Department of Biomedicine, University of Bergen, Jonas Liesvei 91, Bergen, N-5009, Norway

    Stein O Doskeland

Authors
  1. Heiko Poppe
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  2. Sergei D Rybalkin
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  8. Hans-Gottfried Genieser
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  11. Joseph A Beavo
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Corresponding author

Correspondence to Joseph A Beavo.

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Competing interests

H.G.G. is CEO and F.S. is head of Research and Design of BIOLOG Life Science Institute, which sells cyclic nucleotide analogs for research purposes.

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Supplementary Figures 1–5, Supplementary Tables 1–2, Supplementary Methods and Discussion (PDF 629 kb)

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Poppe, H., Rybalkin, S., Rehmann, H. et al. Cyclic nucleotide analogs as probes of signaling pathways. Nat Methods 5, 277–278 (2008). https://doi.org/10.1038/nmeth0408-277

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