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Role of integrin αVβ3 in the production of recombinant adenoviruses in HEK-293 cells

Gene Therapy volume 9, pages 907–914 (2002)Cite this article

Abstract

Adenoviral infection is initiated by attachment of adenoviral fiber proteins to the CAR protein and subsequent internalization aided by αV -containing integrins, eg αVβ3 and αVβ5. To further understand the process of infection and assembly of recombinant adenoviral (rAd) vectors, we examined rAd production in HEK-293 cells and one of its subclones, clone D, isolated from the parental cells for high viral production. By flow cytometry, surface expression of integrin αVβ3 by clone D cells was two-fold higher than by HEK-293 cells. However, clone D cells did not demonstrate greater translational efficiency or number of viral genome DNA copies shortly after rAd infection. Treating cells with inhibitors of integrin αVβ3 reduced rAd production and transfecting HEK-293 cells with integrin αVβ3 cDNAs increased rAd production. Subjecting cells to a sudden reduction in serum (10% to 0.1% FCS) for 5 days, clone D cells maintained 80% viability compared with 40% for HEK-293 cells. Further indication of survival signaling involvement was provided by Western blot analysis demonstrating p38 and p44/42 MAPKs were constitutively phosphorylated in HEK-293 cells. However, for clone D cells, p38 MAPK was phosphorylated only after rAd infection. The role of survival signaling mediated by integrin αVβ3 in rAd production will be discussed.

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Acknowledgements

We would like to thank Dr Mary Ann Fritz for the HPLC analysis, Dr Xiao-Yan Cai for the quantitative PCR analysis. Integrin cDNAs were generous gift of Dr Chandra Kumar and CAR cDNA was a generous gift of Dr Monica Zepeda and Dr William Demers. Anti-CAR antibody was a generous gift of Dr Barry Sugarman.

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Author notes

  1. L Armstrong

    Present address: Aventis Pharmaceuticals, Medical Affairs Oncology, PO Box 6977, 300 Somerset Corporate Blvd, Bridgewater, 08807, NJ, USA

  2. EJ Schaefer

    Present address: Bristol-Myers Squibb Company, Biopharmaceutical Science and Technology, PO Box 5400, Princeton, 08543, NJ, USA

Authors and Affiliations

  1. Department of Biotechnology Development, Schering-Plough Research Institute, Union, NJ, USA

    WLW Ling, RL Longley, DL Brassard & EJ Schaefer

  2. Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, NJ, USA

    L Armstrong

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Ling, W., Longley, R., Brassard, D. et al. Role of integrin αVβ3 in the production of recombinant adenoviruses in HEK-293 cells. Gene Ther 9, 907–914 (2002). https://doi.org/10.1038/sj.gt.3301726

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